LOCUS       BBa_K137131             2923 bp    DNA     linear       03-May-2024
DEFINITION  Lactose inducible lysis cassette expression
ACCESSION   BBa_K137131
VERSION     BBa_K137131.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     misc_feature    44..1351
                     /label=BBa_P0412
     promoter        1360..1462
                     /label=BBa_K137125
     misc_feature    44..1462
                     /label=BBa_S04105
     misc_feature    1..1462
                     /label=BBa_S04106
     misc_feature    1471..2786
                     /label=BBa_K137126
     stem_loop       2806..2849
                     /label=stem_loop
     misc_feature    2795..2874
                     /label=BBa_B0010
     terminator      2795..2874
                     /label=BBa_B0010
     stem_loop       2890..2909
                     /label=T7 TE
     misc_feature    2916..2916
                     /label=stop
     polyA_site      2910..2923
                     /label=polya
     misc_feature    2883..2923
                     /label=BBa_B0012
     terminator      2883..2923
                     /label=BBa_B0012
     terminator      2795..2923
                     /label=BBa_B0015
     misc_feature    1471..2923
                     /label=BBa_S04114
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagt acaacgtcgt gttagttgca
     1381 attgtgagcg gataacaatt gacttgtgag cggataacaa tgatacttcg tgcaattttt
     1441 aaaattaaag gcgttaccca actactagag gattgttcta tcagtaatcg accttattcc
     1501 taattaaata gagcaaatcc ccttattggg ggtaagacat gaagatgcca gaaaaacatg
     1561 acctgttggc cgccattctc gcggcaaagg aacaaggcat cggggcaatc cttgcgtttg
     1621 caatggcgta ccttcgcggc agatataatg gcggtgcgtt tacaaaaaca gtaatcgacg
     1681 caacgatgtg cgccattatc gcctggttca ttcgtgacct tctcgacttc gccggactaa
     1741 gtagcaatct cgcttatata acgagcgtgt ttatcggcta catcggtact gactcgattg
     1801 gttcgcttat caaacgcttc gctgctaaaa aagccggagt agaagatggt agaaatcaat
     1861 aatcaacgta aggcgttcct cgatatgctg gcgtggtcgg agggaactga taacggacgt
     1921 cagaaaacca gaaatcatgg ttatgacgtc attgtaggcg gagagctatt tactgattac
     1981 tccgatcacc ctcgcaaact tgtcacgcta aacccaaaac tcaaatcaac aggcgccgga
     2041 cgctaccagc ttctttcccg ttggtgggat gcctaccgca agcagcttgg cctgaaagac
     2101 ttctctccga aaagtcagga cgctgtggca ttgcagcaga ttaaggagcg tggcgcttta
     2161 cctatgattg atcgtggtga tatccgtcag gcaatcgacc gttgcagcaa tatctgggct
     2221 ttactgccgg gcgctggtta tggtcagttc gagcataagg ctgacagcct gattgcaaaa
     2281 ttcaaagaag cgggcggaac ggtcagagag attgatgtat gagcagagtc accgcgatta
     2341 tctccgctct ggttatctgc atcatcgtct gcctgtcatg ggctgttaat cattaccgtg
     2401 ataacgccat tacctacaaa gcccagcgcg acaaaaatgc cagagaactg aagctggcga
     2461 acgcggcaat tactgacatg cagatgcgtc agcgtgatgt tgctgcgctc gatgcaaaat
     2521 acacgaagga gttagctgat gctaaagctg aaaatgatgc tctgcgtgat gatgttgccg
     2581 ctggtcgtcg tcggttgcac atcaaagcag tctgtcagtc agtgcgtgaa gccaccaccg
     2641 cctccggcgt ggataatgca gcctcccccc gactggcaga caccgctgaa cgggattatt
     2701 tcaccctcag agagaggctg atcactatgc aaaaacaact ggaaggaacc cagaagtata
     2761 ttaatgagca gtgcagatag taataatact agagccaggc atcaaataaa acgaaaggct
     2821 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     2881 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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