LOCUS       BBa_K1379005            1703 bp    DNA     linear       08-May-2024
DEFINITION  &#963;<sup>x</sup> Generator  + P<sub>celA</sub>-E0240
ACCESSION   BBa_K1379005
VERSION     BBa_K1379005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..574
                     /label=comX
     misc_feature    62..64
                     /label=start codon
     misc_feature    568..574
                     /label=stop codon
     CDS             62..574
                     /label=BBa_K1379004
     stem_loop       594..637
                     /label=stem_loop
     misc_feature    583..662
                     /label=BBa_B0010
     terminator      583..662
                     /label=BBa_B0010
     stem_loop       678..697
                     /label=T7 TE
     misc_feature    704..704
                     /label=stop
     polyA_site      698..711
                     /label=polya
     misc_feature    671..711
                     /label=BBa_B0012
     terminator      671..711
                     /label=BBa_B0012
     terminator      583..711
                     /label=BBa_B0015
     misc_feature    1..711
                     /label=BBa_K1379006
     promoter        720..819
                     /label=PcelA
     misc_feature    793..800
                     /label=Com-Box
     promoter        720..819
                     /label=BBa_K1379000
     RBS             828..840
                     /label=RBS-3\Weak
     RBS             834..837
                     /label=RBS
     misc_feature    828..840
                     /label=BBa_B0032
     RBS             828..840
                     /label=BBa_B0032
     CDS             847..1566
                     /label=GFP protein
     CDS             847..1566
                     /label=BBa_E0040
     stem_loop       1586..1629
                     /label=stem_loop
     misc_feature    1575..1654
                     /label=BBa_B0010
     terminator      1575..1654
                     /label=BBa_B0010
     stem_loop       1670..1689
                     /label=T7 TE
     misc_feature    1696..1696
                     /label=stop
     polyA_site      1690..1703
                     /label=polya
     misc_feature    1663..1703
                     /label=BBa_B0012
     terminator      1663..1703
                     /label=BBa_B0012
     misc_feature    828..1703
                     /label=BBa_E0240
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgattaaa gaattgtatg aagaagtcca agggactgtt tataagtgta gaaatgaata
      121 ttaccttcat ttatgggaat tgtcggattg ggaccaagaa ggcatgctct gcttacatga
      181 attgattagt agagaagaag gactggtaga cgatattcca cgtttaagga aatatttcaa
      241 aaccaagttt cgaaatcgaa ttttagacta tatccgtaag caggaaagtc agaagcgtag
      301 atacgataaa gaaccctatg aagaagtggg agagatcagt catcgtataa gtgagggggg
      361 tctctggcta gatgattatt atctctttca tgaaacacta agagattata gaaacaaaca
      421 aagtaaagag aaacaagaag aactagaacg cgtcttaagc aatgaacgat ttcgagggcg
      481 tcaaagagta ttaagagact tacgcattgt gtttaaggag tttactatcc gtacccacga
      541 acaaaaactc atctcagaag aggatctgta ataatactag agccaggcat caaataaaac
      601 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      661 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt
      721 tgaccaagga agactatttt gcaaataaat aagcagttga aaagaaattt ttcgactgtt
      781 ttttcttcct cttacgaata atctaagaga ggagaaaatt actagagtca cacaggaaag
      841 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      901 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      961 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     1021 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     1081 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     1141 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     1201 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1261 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1321 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1381 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1441 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1501 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1561 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1621 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1681 gggtgggcct ttctgcgttt ata
//