LOCUS       BBa_K1379007            1763 bp    DNA     linear       29-Apr-2024
DEFINITION  &#963;<sup>x</sup> Generator  + P<sub>comFA</sub>-E0240
ACCESSION   BBa_K1379007
VERSION     BBa_K1379007.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K880005
     CDS             62..574
                     /label=comX
     misc_feature    62..64
                     /label=start codon
     misc_feature    568..574
                     /label=stop codon
     CDS             62..574
                     /label=BBa_K1379004
     stem_loop       594..637
                     /label=stem_loop
     misc_feature    583..662
                     /label=BBa_B0010
     terminator      583..662
                     /label=BBa_B0010
     stem_loop       678..697
                     /label=T7 TE
     misc_feature    704..704
                     /label=stop
     polyA_site      698..711
                     /label=polya
     misc_feature    671..711
                     /label=BBa_B0012
     terminator      671..711
                     /label=BBa_B0012
     terminator      583..711
                     /label=BBa_B0015
     misc_feature    1..711
                     /label=BBa_K1379006
     promoter        720..879
                     /label=PcomFA
     misc_feature    798..805
                     /label=Com-Box
     promoter        720..879
                     /label=BBa_K1379001
     RBS             888..900
                     /label=RBS-3\Weak
     RBS             894..897
                     /label=RBS
     misc_feature    888..900
                     /label=BBa_B0032
     RBS             888..900
                     /label=BBa_B0032
     CDS             907..1626
                     /label=GFP protein
     CDS             907..1626
                     /label=BBa_E0040
     stem_loop       1646..1689
                     /label=stem_loop
     misc_feature    1635..1714
                     /label=BBa_B0010
     terminator      1635..1714
                     /label=BBa_B0010
     stem_loop       1730..1749
                     /label=T7 TE
     misc_feature    1756..1756
                     /label=stop
     polyA_site      1750..1763
                     /label=polya
     misc_feature    1723..1763
                     /label=BBa_B0012
     terminator      1723..1763
                     /label=BBa_B0012
     misc_feature    888..1763
                     /label=BBa_E0240
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgattaaa gaattgtatg aagaagtcca agggactgtt tataagtgta gaaatgaata
      121 ttaccttcat ttatgggaat tgtcggattg ggaccaagaa ggcatgctct gcttacatga
      181 attgattagt agagaagaag gactggtaga cgatattcca cgtttaagga aatatttcaa
      241 aaccaagttt cgaaatcgaa ttttagacta tatccgtaag caggaaagtc agaagcgtag
      301 atacgataaa gaaccctatg aagaagtggg agagatcagt catcgtataa gtgagggggg
      361 tctctggcta gatgattatt atctctttca tgaaacacta agagattata gaaacaaaca
      421 aagtaaagag aaacaagaag aactagaacg cgtcttaagc aatgaacgat ttcgagggcg
      481 tcaaagagta ttaagagact tacgcattgt gtttaaggag tttactatcc gtacccacga
      541 acaaaaactc atctcagaag aggatctgta ataatactag agccaggcat caaataaaac
      601 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      661 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt
      721 ggacttggcc gtcctcttta attgtcctaa attccatgat ttaattgtac taaaaaatca
      781 tctaaagtgc tagtttttac gaataaagaa gtatgaaagt aaatttagat tatctcggtc
      841 gtttatttac tgagaatgaa ttaacagaag aagaacgtct actagagtca cacaggaaag
      901 tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      961 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     1021 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     1081 acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg
     1141 aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
     1201 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
     1261 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
     1321 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
     1381 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     1441 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     1501 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     1561 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa
     1621 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1681 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1741 gggtgggcct ttctgcgttt ata
//