LOCUS       BBa_K1381022            1489 bp    DNA     linear       02-Jun-2024
DEFINITION  B0034-USP45-CFY-X6 his
ACCESSION   BBa_K1381022
VERSION     BBa_K1381022.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034
     misc_feature    13..18
                     /label=TACTAG
     misc_feature    19..99
                     /label=USP45
     misc_feature    100..129
                     /label=(GS)x5
     misc_feature    130..135
                     /label=AgeI-site
     misc_feature    136..138
                     /label=TAA
     misc_feature    1..139
                     /label=BBa_K1381018
     misc_feature    146..148
                     /label=ATG
     misc_feature    149..154
                     /label=NgoMIV-site
     misc_feature    155..1486
                     /label=Colicin FY
     misc_feature    1487..1489
                     /label=Extra TAA
     CDS             146..1489
                     /label=BBa_K1381017
ORIGIN
        1 gaaagaggag aaatactaga tgaaaaagaa aatcattagc gcgatcctga tgtcaaccgt
       61 aatcctcagc gcggcggctc ccctgagcgg cgtctacgcg ggctctggaa gcggtagtgg
      121 gagcgggtct accggttaat actagatggc cggcacggat tataaagacg tggacccgtt
      181 tgaacggcag gttatggaat ctctgggtta tgggtgggtc agtcaaggag aaggatcgtg
      241 gcagaagggg gaaatttcag gtgcgatcga taatgacact atgacggtaa ccgggtggaa
      301 cggtgccatt ccgacatctc cttccggacg cccgcatatc tgggaccaga aaattagcga
      361 gctgaacaac gcgcggcaaa acagtatcga tgacaacgac gacgacgtca cccccaccag
      421 tgatcatgaa aaaaatcaac gtcgtgaagc ctcggccaaa gcgttgaaat atacctcgga
      481 cattgaaagc aaagtgaacg aacaattaaa tgtcatcaac aatgatattc agaaaacaga
      541 ggccgaatat aaagaaaagc aagggggcct gtcagaagtg gataaagcga aatatcagct
      601 tgtcttatct aaactgcaat tacaaaaaca gaatgccaaa atcctgtccg aagaagcgaa
      661 gaagcgccgc gttcacgcgg aatgggaaca gattaacgat gagaatgttc tgaatgcccg
      721 tgatcgccct agcgcggaat ggcgtcagca gcgtgaaatt aacagcaaaa aaagcctcgc
      781 taaactggcc gaagaacgcg ctgttcaaga acaagttgaa cttattcgcc aggggctcgc
      841 ccagctggaa aagaatattc tcgccaataa agagaatgtg aaacgctgga cggaggagat
      901 gaacaaaaac aaggagaagg aagagaacga agttaaagac gcgctcaaat ttaccgctga
      961 tttttacaag acggtcacgg aaaagtatgg cgaaaaatcc tcgaaaattg cacaagaact
     1021 ggccgatgca gctaaaggta aacagattcg gtcagttgat gaggcgttga aggcgttcga
     1081 taagtataaa gatgtcctga ataagaagtt cagcgtcaaa gatcgggaag ctatcgctaa
     1141 ggcactggaa agcgttgatc gcgaactgat ggccaaaaac ttagcgaaat tcagcaaggc
     1201 gtttaattat gtaggaaaaa ccattgatcg ttatgatacc ctgaaagaga ttcaaaaagc
     1261 gttagaaacc aacaactggc gcccagtgtt tgttcaattg gagtccttag ccgtcggacg
     1321 ggccgccggt gccatcaccg ctttcgcctt ctctatcatt atcggcgcgc ctcttggcat
     1381 tttgggcgtg gccctgatca tggcattagt gtcggtgttc gtggatgatc gctttattga
     1441 gaaaatcaac gaaatggtcg gcgtgcacca tcaccatcac cactaataa
//