BBa_K1383001 1 BBa_K1383001 BBa_K1383001 (mCherry- Theoretical strongest RBS) 2014-10-01T11:00:00Z 2015-05-08T01:10:13Z The native RBS sequence was obtained from Methanococcus maripaludis. The mCherry gene was obtained from Clonentech (Discosoma sp.)and was then codon optimized for expression in Methanococcus. In an effort to expand synthetic biology research for Archaea, we have developed protein expression tools to facilitate fluorescence mediated detection of proteins. For our 2014 project we present 3 new BioBrick parts that iGEMers can use readily. All three of our parts are BioBrick compatible. Specifically, we constructed tools consisting of native/ synthetic Methanococcus RBS site(s) upstream of a gene encoding red fluorescent protein-mCherry. Prior to cloning the mCherry gene was codon optimized for expression in Methanoccocus (also, ensuring BioBrick compatibility in the design considerations). The BioBrick part- BBa_K1383001 consists of the theoretical strongest Methanococcus RBS site upstream of the mCherry gene. This fragment was inserted into pSB1C3 plasmid backbone using EcoRI and PstI restriction enzymes. false false _1760_ 0 12767 9 In stock false We have ensured Bio Brick Compatibility for all our parts. false Rachit Jain annotation2429584 1 mCherry range2429584 1 196 906 annotation2429582 1 Promoter range2429582 1 2 175 annotation2429583 1 Theoretical Strongest RBS range2429583 1 185 190 BBa_K1383001_sequence 1 acgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaatactagagtgggaggtgcgctatggtttcaaaaggagaagaagataatatggcaataataaaagaatttatgagatttaaagttcacatggaaggttcagttaatggtcacgaatttgagatagaaggtgaaggtgaaggtagaccatacgaaggtacacagacagcaaaattaaaagttacaaaaggtggtccattaccattcgcatgggatatattatcaccacagtttatgtacggttcaaaagcatacgttaaacacccagcagatataccagattacctcaaattatcatttccagaaggttttaaatgggaaagagttatgaattttgaagatggtggtgttgttacagttacacaggattcatcattacaggatggtgaatttatatacaaagttaaattaagaggtacaaattttccatcagatggtccagttatgcagaaaaaaacaatgggttgggaagcatcatcagaaagaatgtacccagaagatggtgcattaaaaggtgagataaaacagagattaaaattaaaagatggtggtcactacgatgcagaagttaaaacaacatacaaagcaaaaaaaccagttcagttaccaggtgcatacaacgttaatataaaattagatataacatcacacaatgaagattacacaatagttgaacagtacgaaagagcagaaggtagacactcaacaggtggtatggatgaattatacaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z