BBa_K1401009

BBa_K1401009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1401009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Lewis
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:10:16

Transcriptional Unit with pTet-pBad tandem promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1401009_sequence (Version 1)

Description

This transcriptional unit contains the following: pBad-pTet - BCD2 - RFP - B0015. The transcriptional unit is induced by arabinose and atc. This is a Level 1 MoClo part with flanking sites A on the 5' side and site E on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. This transcriptional unit has Kanamycin resistance.

Notes

This part was created using the MoClo assembly method. The tandem promoters were created by combining two promoters together without any additional sequence in between.

Source

Tandem Promoter Reference Paper

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BBa_K1401009/1