LOCUS       BBa_K1401610            1176 bp    DNA     linear       01-Jun-2024
DEFINITION  pLac - BCD2 - YFP
ACCESSION   BBa_K1401610
VERSION     BBa_K1401610.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K783046
     RBS             213..296
                     /label=BCD2
     misc_feature    209..212
                     /label=MoClo Fusion Site B
     misc_feature    297..300
                     /label=MoClo Fusion Site C
     RBS             209..300
                     /label=BBa_K1114107
     misc_feature    309..1039
                     /label=BBa_K1114208
     stem_loop       1059..1102
                     /label=stem_loop
     misc_feature    1048..1127
                     /label=BBa_B0010
     terminator      1048..1127
                     /label=BBa_B0010
     stem_loop       1143..1162
                     /label=T7 TE
     misc_feature    1169..1169
                     /label=stop
     polyA_site      1163..1176
                     /label=polya
     misc_feature    1136..1176
                     /label=BBa_B0012
     terminator      1136..1176
                     /label=BBa_B0012
     terminator      1048..1176
                     /label=BBa_K783020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc
      241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      301 tactagagaa tgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      361 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      421 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      481 ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc
      541 gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      601 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      661 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      721 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      781 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      841 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      901 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      961 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1021 ctgtacaagt aataaaggtt actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1081 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1141 ctggctcacc ttcgggtggg cctttctgcg tttata
//