LOCUS       BBa_K1401612            1368 bp    DNA     linear       01-Jun-2024
DEFINITION  pLac - BCD2 - araC
ACCESSION   BBa_K1401612
VERSION     BBa_K1401612.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=BBa_R0010
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K783046
     RBS             213..296
                     /label=BCD2
     misc_feature    209..212
                     /label=MoClo Fusion Site B
     misc_feature    297..300
                     /label=MoClo Fusion Site C
     RBS             209..300
                     /label=BBa_K1114107
     CDS             309..1231
                     /label=BBa_K1114204
     stem_loop       1251..1294
                     /label=stem_loop
     misc_feature    1240..1319
                     /label=BBa_B0010
     terminator      1240..1319
                     /label=BBa_B0010
     stem_loop       1335..1354
                     /label=T7 TE
     misc_feature    1361..1361
                     /label=stop
     polyA_site      1355..1368
                     /label=polya
     misc_feature    1328..1368
                     /label=BBa_B0012
     terminator      1328..1368
                     /label=BBa_B0012
     terminator      1240..1368
                     /label=BBa_K783020
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagta ctgggcccaa gttcacttaa aaaggagatc
      241 aacaatgaaa gcaattttcg tactgaaaca tcttaatcat gctaaggagg ttttctaatg
      301 tactagagaa tgatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaac
      361 gcccatctgg tggcgggttt aacgccgatt gaggccaatg gttatctcga tttttttatc
      421 gaccgaccgc tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg
      481 gtgaaaaatc agggacgaga atttgtctgc cgaccgggtg atattttgct gttcccgcca
      541 ggagagattc atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt
      601 tactttcgtc cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat
      661 acgggtttct ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa
      721 atcattaacg ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt
      781 gagcaattgt tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat
      841 aatcgggtac gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat
      901 atcgccagcg tcgcacagca tgtttgcctg tcgccgtcgc gtctgtcaca tcttttccgc
      961 cagcagttag ggattagcgt cttaagctgg cgcgaggacc aacgcatcag ccaggcgaag
     1021 ctgcttttga gcactacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac
     1081 gatcaactct atttctcgcg agtatttaaa aaatgcaccg gggccagccc gagcgagttc
     1141 cgtgccggtt gtgaagaaaa agtgaatgat gtagccgtca agttgtcagc tgcaaacgac
     1201 gaaaactacg ctttagtagc ttaataaagg ttactagagc caggcatcaa ataaaacgaa
     1261 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1321 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//