LOCUS BBa_K1405008 2926 bp DNA linear 02-May-2024 DEFINITION A Kill Switch with "memory" time repressed by IPTG ACCESSION BBa_K1405008 VERSION BBa_K1405008.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 CDS 42..1124 /label=BBa_K1088018 stem_loop 1144..1187 /label=stem_loop misc_feature 1133..1212 /label=BBa_B0010 terminator 1133..1212 /label=BBa_B0010 stem_loop 1228..1247 /label=T7 TE misc_feature 1254..1254 /label=stop polyA_site 1248..1261 /label=polya misc_feature 1221..1261 /label=BBa_B0012 terminator 1221..1261 /label=BBa_B0012 terminator 1133..1261 /label=BBa_B0015 CDS 1270..1357 /label=end of LacI coding region (inactive) misc_feature 1270..1469 /label=BBa_R0010 misc_feature 1358..1395 /label=CAP binding site promoter 1406..1411 /label=-35 promoter 1430..1435 /label=-10 misc_feature 1435..1469 /label=LacI binding site misc_feature 1442..1442 /label=start promoter 1270..1469 /label=BBa_R0010 CDS 1479..2186 /label=cI lambda misc_feature 2187..2219 /label=LVA misc_feature 2226..2250 /label=Help:Barcodes CDS 1476..2250 /label=BBa_C0051 stem_loop 2270..2313 /label=stem_loop misc_feature 2259..2338 /label=BBa_B0010 terminator 2259..2338 /label=BBa_B0010 stem_loop 2354..2373 /label=T7 TE misc_feature 2380..2380 /label=stop polyA_site 2374..2387 /label=polya misc_feature 2347..2387 /label=BBa_B0012 terminator 2347..2387 /label=BBa_B0012 terminator 2259..2387 /label=BBa_B0015 promoter 2433..2438 /label=-10 promoter 2410..2415 /label=-35 misc_feature 2420..2436 /label=OR1 misc_feature 2396..2412 /label=OR2 misc_feature 2396..2444 /label=BBa_R0051 promoter 2396..2444 /label=BBa_R0051 misc_feature 2784..2789 /label=double TAA added CDS 2451..2789 /label=BBa_K302033 stem_loop 2809..2852 /label=stem_loop misc_feature 2798..2877 /label=BBa_B0010 terminator 2798..2877 /label=BBa_B0010 stem_loop 2893..2912 /label=T7 TE misc_feature 2919..2919 /label=stop polyA_site 2913..2926 /label=polya misc_feature 2886..2926 /label=BBa_B0012 terminator 2886..2926 /label=BBa_B0012 terminator 2798..2926 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gatgaaacca gtaacgttat 61 acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg 121 ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt 181 acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg 241 ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg 301 ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct 361 gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc 421 cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat 481 ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta 541 cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg 601 gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc 661 gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc 721 aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg 781 atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg 841 atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa 901 ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac 961 tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa 1021 aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 1081 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgatactag agccaggcat 1141 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1201 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1261 atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 1321 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 1381 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 1441 gaattgtgag cggataacaa tttcacacat actagatgag cacaaaaaag aaaccattaa 1501 cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg 1561 aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg 1621 gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa 1681 ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt 1741 atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc 1801 atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga 1861 gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta 1921 attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg 1981 ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg 2041 agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc 2101 cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta 2161 gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt 2221 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa 2281 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 2341 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtaaca 2401 ccgtgcgtgt tgactatttt acctctggcg gtgataatgg ttgctactag atggtaagcc 2461 gatacgtacc cgatatgggc gatctgattt gggttgattt tgacccgaca aaaggtagcg 2521 agcaagctgg acatcgtcca gctgttgtcc tgagtccttt catgtacaac aacaaaacag 2581 gtatgtgtct gtgtgttcct tgtacaacgc aatcaaaagg atatccgttc gaagttgttt 2641 tatccggtca ggaacgtgat ggcgtagcgt tagctgatca ggtaaaaagt atcgcctggc 2701 gggcaagagg agcaacgaag aaaggaacag ttgccccaga ggaattacaa ctcattaaag 2761 ccaaaattaa cgtactgatt gggtaataat actagagcca ggcatcaaat aaaacgaaag 2821 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2881 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //