LOCUS       BBa_K1407005            1029 bp    DNA     linear       04-May-2024
DEFINITION  LasR/PAI1 Inducible promoter +RBS(B0030)+RFP+Terminator
ACCESSION   BBa_K1407005
VERSION     BBa_K1407005.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=OP2
     misc_feature    106..125
                     /label=OP1
     promoter        117..122
                     /label=-35
     promoter        140..145
                     /label=-10
     promoter        1..157
                     /label=BBa_R0079
     RBS             166..180
                     /label=RBS-1\Strong
     RBS             173..177
                     /label=RBS
     misc_feature    166..180
                     /label=BBa_B0030
     RBS             166..180
                     /label=BBa_B0030
     CDS             187..861
                     /label=mrfp1
     misc_feature    868..892
                     /label=Help:Barcodes
     CDS             187..892
                     /label=BBa_E1010
     misc_feature    1..892
                     /label=BBa_K575010
     stem_loop       912..955
                     /label=stem_loop
     misc_feature    901..980
                     /label=BBa_B0010
     terminator      901..980
                     /label=BBa_B0010
     stem_loop       996..1015
                     /label=T7 TE
     misc_feature    1022..1022
                     /label=stop
     polyA_site      1016..1029
                     /label=polya
     misc_feature    989..1029
                     /label=BBa_B0012
     terminator      989..1029
                     /label=BBa_B0012
     terminator      901..1029
                     /label=BBa_B0015
ORIGIN
        1 gcccctcgct gagcgcgtcc cggagctggg ggcaacctag ctgccacctg cttttctgct
       61 agctattcca gcgaaaacat acagatttcc ggcgaaatca aggctacctg ccagttctgg
      121 caggtttggc cgcgggttct ttttggtaca cgaaagctac tagagattaa agaggagaaa
      181 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
      241 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
      301 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
      361 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
      421 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
      481 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
      541 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
      601 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
      661 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
      721 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
      781 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
      841 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gctactagag
      901 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      961 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1021 gcgtttata
//