LOCUS       BBa_K1407007            1817 bp    DNA     linear       06-May-2024
DEFINITION  RhlR protein(LVA-) +RhlR/PAI2 Inducible promoter 
            +RBS(B0030)+RFP+Terminator
ACCESSION   BBa_K1407007
VERSION     BBa_K1407007.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..747
                     /label=BBa_C0171
     stem_loop       767..810
                     /label=stem_loop
     misc_feature    756..835
                     /label=BBa_B0010
     terminator      756..835
                     /label=BBa_B0010
     stem_loop       851..870
                     /label=T7 TE
     misc_feature    877..877
                     /label=stop
     polyA_site      871..884
                     /label=polya
     misc_feature    844..884
                     /label=BBa_B0012
     terminator      844..884
                     /label=BBa_B0012
     misc_feature    1..884
                     /label=BBa_I1466
     misc_feature    893..912
                     /label=RhlR
     promoter        911..916
                     /label=-35
     promoter        934..939
                     /label=-10
     misc_feature    945..945
                     /label=start
     promoter        893..945
                     /label=BBa_R0071
     RBS             954..968
                     /label=RBS-1\Strong
     RBS             961..965
                     /label=RBS
     misc_feature    954..968
                     /label=BBa_B0030
     RBS             954..968
                     /label=BBa_B0030
     CDS             975..1649
                     /label=mrfp1
     misc_feature    1656..1680
                     /label=Help:Barcodes
     CDS             975..1680
                     /label=BBa_E1010
     misc_feature    893..1680
                     /label=BBa_K575014
     stem_loop       1700..1743
                     /label=stem_loop
     misc_feature    1689..1768
                     /label=BBa_B0010
     terminator      1689..1768
                     /label=BBa_B0010
     stem_loop       1784..1803
                     /label=T7 TE
     misc_feature    1810..1810
                     /label=stop
     polyA_site      1804..1817
                     /label=polya
     misc_feature    1777..1817
                     /label=BBa_B0012
     terminator      1777..1817
                     /label=BBa_B0012
     terminator      1689..1817
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gaggaatgac ggaggctttt tgctgtggtg ggacggtttg
       61 cgtagcgaga tgcagccgat ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa
      121 gtgcggcgcc tgggcttcga ttactacgcc tatggcgtgc gccacacgat tcccttcacc
      181 cggccgaaga ccgaggtcca tggcacctat cccaaggcct ggctggagcg ataccagatg
      241 cagaactacg gggccgtgga tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg
      301 gtctggagcg acagcctgtt cgaccagagc cggatgctct ggaacgaggc tcgcgattgg
      361 ggcctctgtg tcggcgcgac cttgccgatc cgcgcgccga acaatttgct cagcgtgctt
      421 tccgtggcgc gcgaccagca gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg
      481 ctgcgttgca tgatcgagtt gctgacccag aagctgaccg acctggagca tccgatgctg
      541 atgtccaacc cggtctgcct gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac
      601 ggcaagagtt ccggggaaat cgccatcatc ctgagcatct ccgagagcac ggtgaacttc
      661 caccacaaga acatccagaa gaagttcgac gcgccgaaca agacgctggc tgccgcctac
      721 gccgcggcgc tgggtctcat ctaataatac tagagccagg catcaaataa aacgaaaggc
      781 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      841 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agtcctgtga
      901 aatctggcag ttaccgttag ctttcgaatt ggctaaaaag tgttctacta gagattaaag
      961 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
     1021 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
     1081 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
     1141 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
     1201 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
     1261 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
     1321 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
     1381 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
     1441 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
     1501 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
     1561 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
     1621 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc
     1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1801 gcctttctgc gtttata
//