LOCUS       BBa_K1407010            1958 bp    DNA     linear       03-May-2024
DEFINITION  constitutive production of LasR+LasR/PAI1 Inducible promoter 
            +RBS(B0030)+RFP+Terminator
ACCESSION   BBa_K1407010
VERSION     BBa_K1407010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=BBa_C0179
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    1..921
                     /label=BBa_K553003
     misc_feature    975..992
                     /label=OP2
     misc_feature    1035..1054
                     /label=OP1
     promoter        1046..1051
                     /label=-35
     promoter        1069..1074
                     /label=-10
     promoter        930..1086
                     /label=BBa_R0079
     RBS             1095..1109
                     /label=RBS-1\Strong
     RBS             1102..1106
                     /label=RBS
     misc_feature    1095..1109
                     /label=BBa_B0030
     RBS             1095..1109
                     /label=BBa_B0030
     CDS             1116..1790
                     /label=mrfp1
     misc_feature    1797..1821
                     /label=Help:Barcodes
     CDS             1116..1821
                     /label=BBa_E1010
     misc_feature    930..1821
                     /label=BBa_K575010
     stem_loop       1841..1884
                     /label=stem_loop
     misc_feature    1830..1909
                     /label=BBa_B0010
     terminator      1830..1909
                     /label=BBa_B0010
     stem_loop       1925..1944
                     /label=T7 TE
     misc_feature    1951..1951
                     /label=stop
     polyA_site      1945..1958
                     /label=polya
     misc_feature    1918..1958
                     /label=BBa_B0012
     terminator      1918..1958
                     /label=BBa_B0012
     terminator      1830..1958
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg
      961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg
     1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac
     1081 gaaagctact agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
     1141 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
     1201 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
     1261 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
     1321 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
     1381 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
     1441 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
     1501 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
     1561 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
     1621 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1681 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1741 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1801 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1921 cactggctca ccttcgggtg ggcctttctg cgtttata
//