LOCUS       BBa_K1407011            1918 bp    DNA     linear       14-May-2024
DEFINITION  constitutive promoter+RhlR protein generator+RhlR/PAI2 Inducible 
            promoter+RBS(B0030)+RFP+Terminator
ACCESSION   BBa_K1407011
VERSION     BBa_K1407011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=rhlR
     misc_feature    785..817
                     /label=LVA
     misc_feature    824..848
                     /label=Help:Barcodes
     CDS             62..823
                     /label=BBa_C0071
     stem_loop       868..911
                     /label=stem_loop
     misc_feature    857..936
                     /label=BBa_B0010
     terminator      857..936
                     /label=BBa_B0010
     stem_loop       952..971
                     /label=T7 TE
     misc_feature    978..978
                     /label=stop
     polyA_site      972..985
                     /label=polya
     misc_feature    945..985
                     /label=BBa_B0012
     terminator      945..985
                     /label=BBa_B0012
     misc_feature    44..985
                     /label=BBa_I0466
     misc_feature    994..1013
                     /label=RhlR
     promoter        1012..1017
                     /label=-35
     promoter        1035..1040
                     /label=-10
     misc_feature    1046..1046
                     /label=start
     promoter        994..1046
                     /label=BBa_R0071
     RBS             1055..1069
                     /label=RBS-1\Strong
     RBS             1062..1066
                     /label=RBS
     misc_feature    1055..1069
                     /label=BBa_B0030
     RBS             1055..1069
                     /label=BBa_B0030
     CDS             1076..1750
                     /label=mrfp1
     misc_feature    1757..1781
                     /label=Help:Barcodes
     CDS             1076..1781
                     /label=BBa_E1010
     misc_feature    994..1781
                     /label=BBa_K575014
     stem_loop       1801..1844
                     /label=stem_loop
     misc_feature    1790..1869
                     /label=BBa_B0010
     terminator      1790..1869
                     /label=BBa_B0010
     stem_loop       1885..1904
                     /label=T7 TE
     misc_feature    1911..1911
                     /label=stop
     polyA_site      1905..1918
                     /label=polya
     misc_feature    1878..1918
                     /label=BBa_B0012
     terminator      1878..1918
                     /label=BBa_B0012
     terminator      1790..1918
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgaggaat gacggaggct ttttgctgtg gtgggacggt ttgcgtagcg agatgcagcc
      121 gatccacgac agccagggcg tgttcgccgt cctggaaaag gaagtgcggc gcctgggctt
      181 cgattactac gcctatggcg tgcgccacac gattcccttc acccggccga agaccgaggt
      241 ccatggcacc tatcccaagg cctggctgga gcgataccag atgcagaact acggggccgt
      301 ggatccggcg atcctcaacg gcctgcgctc ctcggaaatg gtggtctgga gcgacagcct
      361 gttcgaccag agccggatgc tctggaacga ggctcgcgat tggggcctct gtgtcggcgc
      421 gaccttgccg atccgcgcgc cgaacaattt gctcagcgtg ctttccgtgg cgcgcgacca
      481 gcagaacatc tccagcttcg agcgcgagga aatccgcctg cggctgcgtt gcatgatcga
      541 gttgctgacc cagaagctga ccgacctgga gcatccgatg ctgatgtcca acccggtctg
      601 cctgagccat cgcgaacgcg agatcctgca atggaccgcc gacggcaaga gttccgggga
      661 aatcgccatc atcctgagca tctccgagag cacggtgaac ttccaccaca agaacatcca
      721 gaagaagttc gacgcgccga acaagacgct ggctgccgcc tacgccgcgg cgctgggtct
      781 catcgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg
      841 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatatacta gagtcctgtg aaatctggca gttaccgtta
     1021 gctttcgaat tggctaaaaa gtgttctact agagattaaa gaggagaaat actagatggc
     1081 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
     1141 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
     1201 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
     1261 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
     1321 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
     1381 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
     1441 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
     1501 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
     1561 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
     1621 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
     1681 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
     1741 caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa
     1801 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1861 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//