LOCUS BBa_K1407014 3826 bp DNA linear 03-May-2024 DEFINITION LasR+LasR/PAI1 Inducible promoter+RFP+RhlR(LVA-)+RhlR/PAI2 Inducible promoter+RFP ACCESSION BBa_K1407014 VERSION BBa_K1407014.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..784 /label=BBa_C0179 stem_loop 804..847 /label=stem_loop misc_feature 793..872 /label=BBa_B0010 terminator 793..872 /label=BBa_B0010 stem_loop 888..907 /label=T7 TE misc_feature 914..914 /label=stop polyA_site 908..921 /label=polya misc_feature 881..921 /label=BBa_B0012 terminator 881..921 /label=BBa_B0012 terminator 793..921 /label=BBa_B0015 misc_feature 1..921 /label=BBa_K553003 misc_feature 975..992 /label=OP2 misc_feature 1035..1054 /label=OP1 promoter 1046..1051 /label=-35 promoter 1069..1074 /label=-10 promoter 930..1086 /label=BBa_R0079 RBS 1095..1109 /label=RBS-1\Strong RBS 1102..1106 /label=RBS misc_feature 1095..1109 /label=BBa_B0030 RBS 1095..1109 /label=BBa_B0030 CDS 1116..1790 /label=mrfp1 misc_feature 1797..1821 /label=Help:Barcodes CDS 1116..1821 /label=BBa_E1010 misc_feature 930..1821 /label=BBa_K575010 stem_loop 1841..1884 /label=stem_loop misc_feature 1830..1909 /label=BBa_B0010 terminator 1830..1909 /label=BBa_B0010 stem_loop 1925..1944 /label=T7 TE misc_feature 1951..1951 /label=stop polyA_site 1945..1958 /label=polya misc_feature 1918..1958 /label=BBa_B0012 terminator 1918..1958 /label=BBa_B0012 terminator 1830..1958 /label=BBa_B0015 misc_feature 1..1958 /label=BBa_K1407010 promoter 1967..2001 /label=BBa_J23119 misc_feature 2014..2017 /label=conserved RBS 2010..2021 /label=BBa_B0034 CDS 2028..2756 /label=BBa_C0171 stem_loop 2776..2819 /label=stem_loop misc_feature 2765..2844 /label=BBa_B0010 terminator 2765..2844 /label=BBa_B0010 stem_loop 2860..2879 /label=T7 TE misc_feature 2886..2886 /label=stop polyA_site 2880..2893 /label=polya misc_feature 2853..2893 /label=BBa_B0012 terminator 2853..2893 /label=BBa_B0012 misc_feature 2010..2893 /label=BBa_I1466 misc_feature 2902..2921 /label=RhlR promoter 2920..2925 /label=-35 promoter 2943..2948 /label=-10 misc_feature 2954..2954 /label=start promoter 2902..2954 /label=BBa_R0071 RBS 2963..2977 /label=RBS-1\Strong RBS 2970..2974 /label=RBS misc_feature 2963..2977 /label=BBa_B0030 RBS 2963..2977 /label=BBa_B0030 CDS 2984..3658 /label=mrfp1 misc_feature 3665..3689 /label=Help:Barcodes CDS 2984..3689 /label=BBa_E1010 misc_feature 2902..3689 /label=BBa_K575014 stem_loop 3709..3752 /label=stem_loop misc_feature 3698..3777 /label=BBa_B0010 terminator 3698..3777 /label=BBa_B0010 stem_loop 3793..3812 /label=T7 TE misc_feature 3819..3819 /label=stop polyA_site 3813..3826 /label=polya misc_feature 3786..3826 /label=BBa_B0012 terminator 3786..3826 /label=BBa_B0012 terminator 3698..3826 /label=BBa_B0015 misc_feature 1967..3826 /label=BBa_K1407012 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta 781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg 961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg 1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac 1081 gaaagctact agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca 1141 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 1201 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 1261 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 1321 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 1381 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 1441 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 1501 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 1561 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 1621 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 1681 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 1741 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 1801 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1861 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 1921 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cagctagctc 1981 agtcctaggt ataatgctag ctactagaga aagaggagaa atactagatg aggaatgacg 2041 gaggcttttt gctgtggtgg gacggtttgc gtagcgagat gcagccgatc cacgacagcc 2101 agggcgtgtt cgccgtcctg gaaaaggaag tgcggcgcct gggcttcgat tactacgcct 2161 atggcgtgcg ccacacgatt cccttcaccc ggccgaagac cgaggtccat ggcacctatc 2221 ccaaggcctg gctggagcga taccagatgc agaactacgg ggccgtggat ccggcgatcc 2281 tcaacggcct gcgctcctcg gaaatggtgg tctggagcga cagcctgttc gaccagagcc 2341 ggatgctctg gaacgaggct cgcgattggg gcctctgtgt cggcgcgacc ttgccgatcc 2401 gcgcgccgaa caatttgctc agcgtgcttt ccgtggcgcg cgaccagcag aacatctcca 2461 gcttcgagcg cgaggaaatc cgcctgcggc tgcgttgcat gatcgagttg ctgacccaga 2521 agctgaccga cctggagcat ccgatgctga tgtccaaccc ggtctgcctg agccatcgcg 2581 aacgcgagat cctgcaatgg accgccgacg gcaagagttc cggggaaatc gccatcatcc 2641 tgagcatctc cgagagcacg gtgaacttcc accacaagaa catccagaag aagttcgacg 2701 cgccgaacaa gacgctggct gccgcctacg ccgcggcgct gggtctcatc taataatact 2761 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 2821 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 2881 ttctgcgttt atatactaga gtcctgtgaa atctggcagt taccgttagc tttcgaattg 2941 gctaaaaagt gttctactag agattaaaga ggagaaatac tagatggctt cctccgaaga 3001 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga 3061 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact 3121 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca 3181 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc 3241 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac 3301 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg 3361 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc 3421 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa 3481 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc 3541 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga 3601 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta 3661 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag 3721 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 3781 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //