LOCUS BBa_K1407016 3843 bp DNA linear 05-May-2024 DEFINITION LasR+LasR/PAI1 Inducible promoter+GFP+RhlR(LVA-)+RhlR/PAI2 Inducible promoter+RFP ACCESSION BBa_K1407016 VERSION BBa_K1407016.1 FEATURES Location/Qualifiers misc_feature 1..35 /label=BBa_J23100 misc_feature 1..921 /label=BBa_K553003 misc_feature 44..55 /label=BBa_B0034 misc_feature 48..51 /label=conserved misc_feature 62..784 /label=BBa_C0179 misc_feature 793..921 /label=BBa_B0015 misc_feature 793..872 /label=BBa_B0010 stem_loop 804..847 /label=stem_loop misc_feature 881..921 /label=BBa_B0012 stem_loop 888..907 /label=T7 TE polyA_site 908..921 /label=polya misc_feature 914..914 /label=stop misc_feature 930..1086 /label=BBa_R0079 misc_feature 930..1832 /label=BBa_K575009 misc_feature 975..992 /label=OP2 misc_feature 1035..1054 /label=OP1 promoter 1046..1051 /label=-35 promoter 1069..1074 /label=-10 misc_feature 1095..1106 /label=BBa_B0034 misc_feature 1099..1102 /label=conserved CDS 1113..1832 /label=GFP protein misc_feature 1113..1832 /label=BBa_E0040 misc_feature 1841..1920 /label=BBa_B0010 misc_feature 1841..1969 /label=BBa_B0015 stem_loop 1852..1895 /label=stem_loop misc_feature 1929..1969 /label=BBa_B0012 stem_loop 1936..1955 /label=T7 TE polyA_site 1956..1969 /label=polya misc_feature 1962..1962 /label=stop misc_feature 1..1969 /label=BBa_K1407008 misc_feature 1978..2012 /label=BBa_J23119 misc_feature 2021..2032 /label=BBa_B0034 misc_feature 2021..2904 /label=BBa_I1466 misc_feature 2025..2028 /label=conserved misc_feature 2039..2767 /label=BBa_C0171 misc_feature 2776..2855 /label=BBa_B0010 stem_loop 2787..2830 /label=stem_loop misc_feature 2864..2904 /label=BBa_B0012 stem_loop 2871..2890 /label=T7 TE polyA_site 2891..2904 /label=polya misc_feature 2897..2897 /label=stop misc_feature 2913..2932 /label=RhlR misc_feature 2913..2965 /label=BBa_R0071 misc_feature 2913..3700 /label=BBa_K575014 promoter 2931..2936 /label=-35 promoter 2954..2959 /label=-10 misc_feature 2965..2965 /label=start RBS 2974..2988 /label=RBS-1\Strong misc_feature 2974..2988 /label=BBa_B0030 RBS 2981..2985 /label=RBS CDS 2995..3669 /label=mrfp1 misc_feature 2995..3700 /label=BBa_E1010 misc_feature 3676..3700 /label=Help:Barcodes misc_feature 3709..3788 /label=BBa_B0010 misc_feature 3709..3837 /label=BBa_B0015 stem_loop 3720..3763 /label=stem_loop misc_feature 3797..3837 /label=BBa_B0012 stem_loop 3804..3823 /label=T7 TE polyA_site 3824..3837 /label=polya misc_feature 3830..3830 /label=stop misc_feature 1978..3837 /label=BBa_K1407012 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta 781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 901 gtgggccttt ctgcgtttat atactagagg cccctcgctg agcgcgtccc ggagctgggg 961 gcaacctagc tgccacctgc ttttctgcta gctattccag cgaaaacata cagatttccg 1021 gcgaaatcaa ggctacctgc cagttctggc aggtttggcc gcgggttctt tttggtacac 1081 gaaagctact agagaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg 1141 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca 1201 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta 1261 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat 1321 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg 1381 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg 1441 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt 1501 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg 1561 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca 1621 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg 1681 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag 1741 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta 1801 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga 1861 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1921 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagttg 1981 acagctagct cagtcctagg tataatgcta gctactagag aaagaggaga aatactagat 2041 gaggaatgac ggaggctttt tgctgtggtg ggacggtttg cgtagcgaga tgcagccgat 2101 ccacgacagc cagggcgtgt tcgccgtcct ggaaaaggaa gtgcggcgcc tgggcttcga 2161 ttactacgcc tatggcgtgc gccacacgat tcccttcacc cggccgaaga ccgaggtcca 2221 tggcacctat cccaaggcct ggctggagcg ataccagatg cagaactacg gggccgtgga 2281 tccggcgatc ctcaacggcc tgcgctcctc ggaaatggtg gtctggagcg acagcctgtt 2341 cgaccagagc cggatgctct ggaacgaggc tcgcgattgg ggcctctgtg tcggcgcgac 2401 cttgccgatc cgcgcgccga acaatttgct cagcgtgctt tccgtggcgc gcgaccagca 2461 gaacatctcc agcttcgagc gcgaggaaat ccgcctgcgg ctgcgttgca tgatcgagtt 2521 gctgacccag aagctgaccg acctggagca tccgatgctg atgtccaacc cggtctgcct 2581 gagccatcgc gaacgcgaga tcctgcaatg gaccgccgac ggcaagagtt ccggggaaat 2641 cgccatcatc ctgagcatct ccgagagcac ggtgaacttc caccacaaga acatccagaa 2701 gaagttcgac gcgccgaaca agacgctggc tgccgcctac gccgcggcgc tgggtctcat 2761 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2821 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2881 cgggtgggcc tttctgcgtt tatatactag agtcctgtga aatctggcag ttaccgttag 2941 ctttcgaatt ggctaaaaag tgttctacta gagattaaag aggagaaata ctagatggct 3001 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt 3061 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag 3121 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc 3181 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac 3241 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt 3301 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt 3361 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt 3421 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa 3481 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg 3541 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc 3601 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc 3661 accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa 3721 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 3781 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 3841 tag //