LOCUS       BBa_K1412005             800 bp    DNA     linear       01-Jun-2024
DEFINITION  RBS (1.0) CheZ double terminators
ACCESSION   BBa_K1412005
VERSION     BBa_K1412005.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     primer_bind     19..38
                     /label=fwd primer
     misc_feature    19..21
                     /label=start
     primer_bind     642..663
                     /label=rev primer
     misc_feature    661..663
                     /label=terminator
     CDS             19..663
                     /label=CheZ
     misc_feature    661..663
                     /label=TGA to TAA
     CDS             19..663
                     /label=BBa_K629003
     stem_loop       683..726
                     /label=stem_loop
     misc_feature    672..751
                     /label=BBa_B0010
     terminator      672..751
                     /label=BBa_B0010
     stem_loop       767..786
                     /label=T7 TE
     misc_feature    793..793
                     /label=stop
     polyA_site      787..800
                     /label=polya
     misc_feature    760..800
                     /label=BBa_B0012
     terminator      760..800
                     /label=BBa_B0012
     terminator      672..800
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gatgcaacca tcaatcaaac ctgctgacga gcattcagct
       61 ggcgatatca ttgcgcgcat cggcagcctg acgcgtatgc tgcgcgacag tttgcgggaa
      121 ctggggctgg atcaggccat tgccgaagcg gcggaagcca tccccgatgc gcgcgatcgt
      181 ttgtactatg ttgtgcagat gaccgcccag gctgcggagc gggcgctgaa cagtgttgag
      241 gcgtcacaac cgcatcagga tcaaatggag aaatcagcaa aagcgttaac ccaacgttgg
      301 gatgactggt ttgccgatcc gattgacctt gccgacgccc gtgaactggt aacagataca
      361 cgacaatttc tggcagatgt acccgcgcat accagcttta ctaacgcgca actgctggaa
      421 atcatgatgg cgcaggattt tcaggatctc accgggcagg tcattaagcg gatgatggat
      481 gtcattcagg agatcgaacg ccagttgctg atggtgctgt tggaaaacat cccggaacag
      541 gagtcgcgtc caaaacgtga aaaccagagt ttgcttaatg gacctcaggt cgataccagc
      601 aaagccggtg tggtagccag tcaggatcag gtggacgatt tgttggatag tcttggattt
      661 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      721 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      781 tgggcctttc tgcgtttata
//