LOCUS       BBa_K1412008             886 bp    DNA     linear       17-May-2024
DEFINITION  Drive the E.coli engineered strain(CL-1) towards death region
ACCESSION   BBa_K1412008
VERSION     BBa_K1412008.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    37..44
                     /label=linker
     promoter        45..50
                     /label=-35
     promoter        68..73
                     /label=-10
     misc_feature    41..41
                     /label=A
     promoter        1..78
                     /label=BBa_R0083
     misc_feature    91..94
                     /label=conserved
     RBS             87..98
                     /label=BBa_B0034
     primer_bind     105..124
                     /label=fwd primer
     misc_feature    105..107
                     /label=start
     primer_bind     728..749
                     /label=rev primer
     misc_feature    747..749
                     /label=terminator
     CDS             105..749
                     /label=CheZ
     misc_feature    747..749
                     /label=TGA to TAA
     CDS             105..749
                     /label=BBa_K629003
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     terminator      758..886
                     /label=BBa_B0015
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgatctct tgagttggat tattctgcat
       61 ttttggggag aatggactta ctagagaaag aggagaaata ctagatgatg caaccatcaa
      121 tcaaacctgc tgacgagcat tcagctggcg atatcattgc gcgcatcggc agcctgacgc
      181 gtatgctgcg cgacagtttg cgggaactgg ggctggatca ggccattgcc gaagcggcgg
      241 aagccatccc cgatgcgcgc gatcgtttgt actatgttgt gcagatgacc gcccaggctg
      301 cggagcgggc gctgaacagt gttgaggcgt cacaaccgca tcaggatcaa atggagaaat
      361 cagcaaaagc gttaacccaa cgttgggatg actggtttgc cgatccgatt gaccttgccg
      421 acgcccgtga actggtaaca gatacacgac aatttctggc agatgtaccc gcgcatacca
      481 gctttactaa cgcgcaactg ctggaaatca tgatggcgca ggattttcag gatctcaccg
      541 ggcaggtcat taagcggatg atggatgtca ttcaggagat cgaacgccag ttgctgatgg
      601 tgctgttgga aaacatcccg gaacaggagt cgcgtccaaa acgtgaaaac cagagtttgc
      661 ttaatggacc tcaggtcgat accagcaaag ccggtgtggt agccagtcag gatcaggtgg
      721 acgatttgtt ggatagtctt ggattttaat actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//