LOCUS       BBa_K1412009            2319 bp    DNA     linear       17-May-2024
DEFINITION  asddff
ACCESSION   BBa_K1412009
VERSION     BBa_K1412009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1166
                     /label=BBa_I732100
     stem_loop       1186..1229
                     /label=stem_loop
     misc_feature    1175..1254
                     /label=BBa_B0010
     terminator      1175..1254
                     /label=BBa_B0010
     stem_loop       1270..1289
                     /label=T7 TE
     misc_feature    1296..1296
                     /label=stop
     polyA_site      1290..1303
                     /label=polya
     misc_feature    1263..1303
                     /label=BBa_B0012
     terminator      1263..1303
                     /label=BBa_B0012
     misc_feature    63..1303
                     /label=BBa_I732820
     CDS             1312..1399
                     /label=end of LacI coding region (inactive)
     misc_feature    1312..1511
                     /label=BBa_R0010
     misc_feature    1400..1437
                     /label=CAP binding site
     promoter        1448..1453
                     /label=-35
     promoter        1472..1477
                     /label=-10
     misc_feature    1477..1511
                     /label=LacI binding site
     misc_feature    1484..1484
                     /label=start
     promoter        1312..1511
                     /label=BBa_R0010
     misc_feature    1524..1527
                     /label=conserved
     RBS             1520..1531
                     /label=BBa_B0034
     primer_bind     1538..1557
                     /label=fwd primer
     misc_feature    1538..1540
                     /label=start
     primer_bind     2161..2182
                     /label=rev primer
     misc_feature    2180..2182
                     /label=terminator
     CDS             1538..2182
                     /label=CheZ
     misc_feature    2180..2182
                     /label=TGA to TAA
     CDS             1538..2182
                     /label=BBa_K629003
     stem_loop       2202..2245
                     /label=stem_loop
     misc_feature    2191..2270
                     /label=BBa_B0010
     terminator      2191..2270
                     /label=BBa_B0010
     stem_loop       2286..2305
                     /label=T7 TE
     misc_feature    2312..2312
                     /label=stop
     polyA_site      2306..2319
                     /label=polya
     misc_feature    2279..2319
                     /label=BBa_B0012
     terminator      2279..2319
                     /label=BBa_B0012
     terminator      2191..2319
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag taataatact agagccaggc atcaaataaa acgaaaggct
     1201 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1261 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gcaatacgca
     1321 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1381 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     1441 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     1501 aatttcacac atactagaga aagaggagaa atactagatg atgcaaccat caatcaaacc
     1561 tgctgacgag cattcagctg gcgatatcat tgcgcgcatc ggcagcctga cgcgtatgct
     1621 gcgcgacagt ttgcgggaac tggggctgga tcaggccatt gccgaagcgg cggaagccat
     1681 ccccgatgcg cgcgatcgtt tgtactatgt tgtgcagatg accgcccagg ctgcggagcg
     1741 ggcgctgaac agtgttgagg cgtcacaacc gcatcaggat caaatggaga aatcagcaaa
     1801 agcgttaacc caacgttggg atgactggtt tgccgatccg attgaccttg ccgacgcccg
     1861 tgaactggta acagatacac gacaatttct ggcagatgta cccgcgcata ccagctttac
     1921 taacgcgcaa ctgctggaaa tcatgatggc gcaggatttt caggatctca ccgggcaggt
     1981 cattaagcgg atgatggatg tcattcagga gatcgaacgc cagttgctga tggtgctgtt
     2041 ggaaaacatc ccggaacagg agtcgcgtcc aaaacgtgaa aaccagagtt tgcttaatgg
     2101 acctcaggtc gataccagca aagccggtgt ggtagccagt caggatcagg tggacgattt
     2161 gttggatagt cttggatttt aatactagag ccaggcatca aataaaacga aaggctcagt
     2221 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2281 acactggctc accttcgggt gggcctttct gcgtttata
//