LOCUS BBa_K1412033 3210 bp DNA linear 17-May-2024 DEFINITION 12345 ACCESSION BBa_K1412033 VERSION BBa_K1412033.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..746 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 terminator 755..883 /label=BBa_B0015 misc_feature 1..883 /label=BBa_K145201 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 CDS 972..2057 /label=BBa_I732100 stem_loop 2077..2120 /label=stem_loop misc_feature 2066..2145 /label=BBa_B0010 terminator 2066..2145 /label=BBa_B0010 stem_loop 2161..2180 /label=T7 TE misc_feature 2187..2187 /label=stop polyA_site 2181..2194 /label=polya misc_feature 2154..2194 /label=BBa_B0012 terminator 2154..2194 /label=BBa_B0012 misc_feature 954..2194 /label=BBa_I732820 CDS 2203..2290 /label=end of LacI coding region (inactive) misc_feature 2203..2402 /label=BBa_R0010 misc_feature 2291..2328 /label=CAP binding site promoter 2339..2344 /label=-35 promoter 2363..2368 /label=-10 misc_feature 2368..2402 /label=LacI binding site misc_feature 2375..2375 /label=start promoter 2203..2402 /label=BBa_R0010 misc_feature 2415..2418 /label=conserved RBS 2411..2422 /label=BBa_B0034 primer_bind 2429..2448 /label=fwd primer misc_feature 2429..2431 /label=start primer_bind 3052..3073 /label=rev primer misc_feature 3071..3073 /label=terminator CDS 2429..3073 /label=CheZ misc_feature 3071..3073 /label=TGA to TAA CDS 2429..3073 /label=BBa_K629003 stem_loop 3093..3136 /label=stem_loop misc_feature 3082..3161 /label=BBa_B0010 terminator 3082..3161 /label=BBa_B0010 stem_loop 3177..3196 /label=T7 TE misc_feature 3203..3203 /label=stop polyA_site 3197..3210 /label=polya misc_feature 3170..3210 /label=BBa_B0012 terminator 3170..3210 /label=BBa_B0012 terminator 3082..3210 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 961 agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 1021 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 1081 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 1141 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 1201 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 1261 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 1321 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 1381 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 1441 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 1501 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 1561 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 1621 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 1681 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 1741 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 1801 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 1861 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 1921 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 1981 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 2041 aaagcgggca gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2101 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2161 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc 2221 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 2281 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 2341 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 2401 catactagag aaagaggaga aatactagat gatgcaacca tcaatcaaac ctgctgacga 2461 gcattcagct ggcgatatca ttgcgcgcat cggcagcctg acgcgtatgc tgcgcgacag 2521 tttgcgggaa ctggggctgg atcaggccat tgccgaagcg gcggaagcca tccccgatgc 2581 gcgcgatcgt ttgtactatg ttgtgcagat gaccgcccag gctgcggagc gggcgctgaa 2641 cagtgttgag gcgtcacaac cgcatcagga tcaaatggag aaatcagcaa aagcgttaac 2701 ccaacgttgg gatgactggt ttgccgatcc gattgacctt gccgacgccc gtgaactggt 2761 aacagataca cgacaatttc tggcagatgt acccgcgcat accagcttta ctaacgcgca 2821 actgctggaa atcatgatgg cgcaggattt tcaggatctc accgggcagg tcattaagcg 2881 gatgatggat gtcattcagg agatcgaacg ccagttgctg atggtgctgt tggaaaacat 2941 cccggaacag gagtcgcgtc caaaacgtga aaaccagagt ttgcttaatg gacctcaggt 3001 cgataccagc aaagccggtg tggtagccag tcaggatcag gtggacgatt tgttggatag 3061 tcttggattt taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 3121 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 3181 caccttcggg tgggcctttc tgcgtttata //