LOCUS       BBa_K1412801            1007 bp    DNA     linear       14-Jun-2024
DEFINITION  Characterize efficiency of RBS with chemotaxis
ACCESSION   BBa_K1412801
VERSION     BBa_K1412801.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     primer_bind     226..245
                     /label=fwd primer
     misc_feature    226..228
                     /label=start
     primer_bind     849..870
                     /label=rev primer
     misc_feature    868..870
                     /label=terminator
     CDS             226..870
                     /label=CheZ
     misc_feature    868..870
                     /label=TGA to TAA
     CDS             226..870
                     /label=BBa_K629003
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    879..958
                     /label=BBa_B0010
     terminator      879..958
                     /label=BBa_B0010
     stem_loop       974..993
                     /label=T7 TE
     misc_feature    1000..1000
                     /label=stop
     polyA_site      994..1007
                     /label=polya
     misc_feature    967..1007
                     /label=BBa_B0012
     terminator      967..1007
                     /label=BBa_B0012
     terminator      879..1007
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatgat gcaaccatca
      241 atcaaacctg ctgacgagca ttcagctggc gatatcattg cgcgcatcgg cagcctgacg
      301 cgtatgctgc gcgacagttt gcgggaactg gggctggatc aggccattgc cgaagcggcg
      361 gaagccatcc ccgatgcgcg cgatcgtttg tactatgttg tgcagatgac cgcccaggct
      421 gcggagcggg cgctgaacag tgttgaggcg tcacaaccgc atcaggatca aatggagaaa
      481 tcagcaaaag cgttaaccca acgttgggat gactggtttg ccgatccgat tgaccttgcc
      541 gacgcccgtg aactggtaac agatacacga caatttctgg cagatgtacc cgcgcatacc
      601 agctttacta acgcgcaact gctggaaatc atgatggcgc aggattttca ggatctcacc
      661 gggcaggtca ttaagcggat gatggatgtc attcaggaga tcgaacgcca gttgctgatg
      721 gtgctgttgg aaaacatccc ggaacaggag tcgcgtccaa aacgtgaaaa ccagagtttg
      781 cttaatggac ctcaggtcga taccagcaaa gccggtgtgg tagccagtca ggatcaggtg
      841 gacgatttgt tggatagtct tggattttaa tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//