LOCUS       BBa_K1412829            1009 bp    DNA     linear       01-Jun-2024
DEFINITION  Characterize efficiency of RBS with chemotaxis
ACCESSION   BBa_K1412829
VERSION     BBa_K1412829.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     primer_bind     228..247
                     /label=fwd primer
     misc_feature    228..230
                     /label=start
     primer_bind     851..872
                     /label=rev primer
     misc_feature    870..872
                     /label=terminator
     CDS             228..872
                     /label=CheZ
     misc_feature    870..872
                     /label=TGA to TAA
     CDS             228..872
                     /label=BBa_K629003
     stem_loop       892..935
                     /label=stem_loop
     misc_feature    881..960
                     /label=BBa_B0010
     terminator      881..960
                     /label=BBa_B0010
     stem_loop       976..995
                     /label=T7 TE
     misc_feature    1002..1002
                     /label=stop
     polyA_site      996..1009
                     /label=polya
     misc_feature    969..1009
                     /label=BBa_B0012
     terminator      969..1009
                     /label=BBa_B0012
     terminator      881..1009
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg atgcaaccat
      241 caatcaaacc tgctgacgag cattcagctg gcgatatcat tgcgcgcatc ggcagcctga
      301 cgcgtatgct gcgcgacagt ttgcgggaac tggggctgga tcaggccatt gccgaagcgg
      361 cggaagccat ccccgatgcg cgcgatcgtt tgtactatgt tgtgcagatg accgcccagg
      421 ctgcggagcg ggcgctgaac agtgttgagg cgtcacaacc gcatcaggat caaatggaga
      481 aatcagcaaa agcgttaacc caacgttggg atgactggtt tgccgatccg attgaccttg
      541 ccgacgcccg tgaactggta acagatacac gacaatttct ggcagatgta cccgcgcata
      601 ccagctttac taacgcgcaa ctgctggaaa tcatgatggc gcaggatttt caggatctca
      661 ccgggcaggt cattaagcgg atgatggatg tcattcagga gatcgaacgc cagttgctga
      721 tggtgctgtt ggaaaacatc ccggaacagg agtcgcgtcc aaaacgtgaa aaccagagtt
      781 tgcttaatgg acctcaggtc gataccagca aagccggtgt ggtagccagt caggatcagg
      841 tggacgattt gttggatagt cttggatttt aatactagag ccaggcatca aataaaacga
      901 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      961 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//