BBa_K1413002 1 BBa_K1413002 P0 promoter-RBS SD001-sfGFP- Terminator B0015 - Pr promoter-DmpR 2014-10-14T11:00:00Z 2015-05-08T01:10:18Z This part derives from BBa_K1413001. This part consist on a sensor of phenolic compounds based on DmpR, a transcription factor of the Ntrc family. Found in Pseudomonas sp. strain CF 600, DmpR regulates expression from the Po promoter, which drives transcription from one single large operon for phenol degradation (dmpKLMNOPQBCDEFGHI). With GFP attached to P0 promoter, it is then possible to evaluate presence of phenol by fluorescence analysis, if DmpR is expressed. This part is basically composed of [P0 promoter + sfGFP + Pr promoter + DmpR] and allows sensing of phenolic compounds thanks to a constitutively expressed DmpR that will activate expression of GFP. We characterized this biosensor using phenol as effector. false false _1791_ 0 22607 9 It's complicated true It has been obtained by performing a mutation of 3 nucleotideS within the sequence of RBS B0032 32. false Nandjafot MENDY annotation2429685 1 sfGFP range2429685 1 199 929 annotation2429684 1 RBS JUNCTION range2429684 1 193 198 annotation2429682 1 RBS JUNCTION range2429682 1 172 179 annotation2429686 1 TERMINATOR range2429686 1 930 1058 annotation2429688 1 PR Promoter range2429688 1 1082 1155 annotation2429689 1 DmpR range2429689 1 1156 2847 annotation2429687 1 SpeI/XbaI mixed site range2429687 1 1059 1066 annotation2429683 1 RBS SD001 range2429683 1 180 192 annotation2429681 1 P0 Promoter range2429681 1 1 171 BBa_K1413002_sequence 1 taagcatttgctcaagcggccttgggcaattgatcaaatgcttaaaaagtctgcgcaagcgcggcttaatttcgctcgctccgatcattctaaaaattagaaacacattgaaaaacattaccttgaagtctgttttcagaccttggcacagccgttgcttgatgtcctgcgtactagagtcaaggaggaaagactagaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggatgaccccatctggaatcgccgcctgccttgcgctatagcggcgaccctgattttccccatctaaaaataaataggggcctcgcttacatgccgatcaagtacaagcctgaaatccagcactccgatttcaaggacctgaccaacctgatccacttccagagcatggaaggcaagatctggcttggcgaacagcgcatgctgttgcttcagttttcagcgatggccagctttcgccgggaaatggtcaataccctgggcatcgaacgcgccaagggcttgttcctgcgccatggttaccagtccggcctgaaggatgccgaactggccaggaagctgagaccgaatgccagcgaagtcggcatgttcctcgctgggccgcagatgcattcactcaagggtctggtcaaggtccgccccaccgagctcgatatcgacaaggaatacgggcgcttctatgccgagatggagtggatcgactccttcgaggtggaaatctgccagaccgacctggggcagatgcaagacccggtgtgctggactctgctcggctacgcctgcgcctattcctcggcgttcatgggccgggaaatcatcttcaaggaagtcagctgcggcggctgcggcggcgacaagtgccgggtcattggcaagccggccgaagagtgggacgacgttgccagcttcaaacagtatttcaagaacgaccccatcatcgaggaactctacgagttgcaatcgcaactgttgtcgctgcgtaccaacctcgacaaacaggaaggccagtactacggcatcggtcagaccccggcctaccagaccgtgcgcaatatgatggacaaggccgcacagggcaaagtctcggtgctgctgcttggcgagaccggggtcggcaaggaggtcatcgcgcgtagcgtgcacctgcgcagcaaacgcgccgccgagccctttgtcgcggtgaactgtgcggcgatcccgccggacctgatcgagtccgaattgttcggcgtggaaaaaggcgccttcaccggcgcctcccagtcacgcatgggccgcttcgagcgggccgacaagggcaccatcttccttgacgaggtgatcgaactcagcccgcgcgctcaggccagtctgctgcgcgtgctgcaagaaggcgagctggagcgagttggcgacaaccgcacgcgcaagatcgacgtaagggttatcgccgccacccacgaggacctggccgaagcggtcaaggccgggcgttttcgcgccgacctgtactaccgactgaacgttttcccggtggcgatcccggcgttgcgcgaacgccgcgaggacattccactgctggttgagcacttccttcagcgcttccaccaggagtacggcaagagaaccctcggcctttcagacaaagccctggaggcctgcctgcattacagttggccgggcaatatccgtgagctggagaacgtcatcgagcgcggcatcatcctcaccgatccgaacgaaagcatcagcgtgcaggcgctgttcctacgggcgccggaagagccgcagaccgccagcgagcgggtgtcgttggacggcgtgctgattcagccaggcaatggccagggcagttggatcagccagttgttgagcagcggcctgagcctcgacgagatcgaggaaagcctgatgcgcgaagccatgcaacaggccaaccaaaacgtctccggtgccgcgcgcttgctcggcctaagccgaccggcactggcctatcggctgaagaaaatcggcatcgaaggctag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z