LOCUS       BBa_K143071             1491 bp    DNA     linear       10-May-2024
DEFINITION  AmyE integratable PoPS generator (P43-gsiB) (with CmR)
ACCESSION   BBa_K143071
VERSION     BBa_K143071.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     promoter        544..552
                     /label=Sigma B -35
     promoter        568..577
                     /label=Sigma B -10
     promoter        554..559
                     /label=Sigma A -35
     promoter        577..582
                     /label=Sigma A -10
     promoter        531..586
                     /label=BBa_K143013
     RBS             596..602
                     /label=rbs
     RBS             595..605
                     /label=BBa_K143020
     misc_feature    612..1271
                     /label=CmR gene
     CDS             612..1271
                     /label=BBa_J31005
     stem_loop       1291..1334
                     /label=stem_loop
     misc_feature    1280..1359
                     /label=BBa_B0010
     terminator      1280..1359
                     /label=BBa_B0010
     stem_loop       1375..1394
                     /label=T7 TE
     misc_feature    1401..1401
                     /label=stop
     polyA_site      1395..1408
                     /label=polya
     misc_feature    1368..1408
                     /label=BBa_B0012
     terminator      1368..1408
                     /label=BBa_B0012
     promoter        1430..1438
                     /label=Sigma B -35
     promoter        1454..1463
                     /label=Sigma B -10
     promoter        1440..1445
                     /label=Sigma A -35
     promoter        1463..1468
                     /label=Sigma A -10
     promoter        1417..1472
                     /label=BBa_K143013
     RBS             1482..1488
                     /label=rbs
     RBS             1481..1491
                     /label=BBa_K143020
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt
      541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagtaaagg
      601 aggaatacta gatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      661 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      721 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      781 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      841 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      901 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      961 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     1021 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     1081 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     1141 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     1201 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     1261 gcggggcgta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1381 ccttcgggtg ggcctttctg cgtttatata ctagagattt tacattttta gaaatgggcg
     1441 tgaaaaaaag cgcgcgatta tgtaaaatat aatactagag taaaggagga a
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