LOCUS       BBa_K143072             1493 bp    DNA     linear       10-May-2024
DEFINITION  AmyE integratable PoPS generator (P43-spoVG) (with CmR)
ACCESSION   BBa_K143072
VERSION     BBa_K143072.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     promoter        544..552
                     /label=Sigma B -35
     promoter        568..577
                     /label=Sigma B -10
     promoter        554..559
                     /label=Sigma A -35
     promoter        577..582
                     /label=Sigma A -10
     promoter        531..586
                     /label=BBa_K143013
     RBS             595..606
                     /label=rbs
     RBS             595..606
                     /label=BBa_K143021
     misc_feature    613..1272
                     /label=CmR gene
     CDS             613..1272
                     /label=BBa_J31005
     stem_loop       1292..1335
                     /label=stem_loop
     misc_feature    1281..1360
                     /label=BBa_B0010
     terminator      1281..1360
                     /label=BBa_B0010
     stem_loop       1376..1395
                     /label=T7 TE
     misc_feature    1402..1402
                     /label=stop
     polyA_site      1396..1409
                     /label=polya
     misc_feature    1369..1409
                     /label=BBa_B0012
     terminator      1369..1409
                     /label=BBa_B0012
     promoter        1431..1439
                     /label=Sigma B -35
     promoter        1455..1464
                     /label=Sigma B -10
     promoter        1441..1446
                     /label=Sigma A -35
     promoter        1464..1469
                     /label=Sigma A -10
     promoter        1418..1473
                     /label=BBa_K143013
     RBS             1482..1493
                     /label=rbs
     RBS             1482..1493
                     /label=BBa_K143021
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt
      541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagaaaggt
      601 ggtgaatact agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      661 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      721 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      781 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      841 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      901 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      961 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1021 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     1081 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1141 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1201 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1261 ggcggggcgt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1321 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1381 accttcgggt gggcctttct gcgtttatat actagagatt ttacattttt agaaatgggc
     1441 gtgaaaaaaa gcgcgcgatt atgtaaaata taatactaga gaaaggtggt gaa
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