LOCUS       BBa_K143074             1575 bp    DNA     linear       10-May-2024
DEFINITION  AmyE integratable PoPS generator (Pveg-spoVG) (with CmR)
ACCESSION   BBa_K143074
VERSION     BBa_K143074.1  
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label=5' AmyE homologous sequence
     misc_feature    1..522
                     /label=BBa_K143001
     promoter        593..598
                     /label=Sigma A-35
     promoter        616..621
                     /label=Sigma A -10
     promoter        531..627
                     /label=BBa_K143012
     RBS             636..647
                     /label=rbs
     RBS             636..647
                     /label=BBa_K143021
     misc_feature    654..1313
                     /label=CmR gene
     CDS             654..1313
                     /label=BBa_J31005
     stem_loop       1333..1376
                     /label=stem_loop
     misc_feature    1322..1401
                     /label=BBa_B0010
     terminator      1322..1401
                     /label=BBa_B0010
     stem_loop       1417..1436
                     /label=T7 TE
     misc_feature    1443..1443
                     /label=stop
     polyA_site      1437..1450
                     /label=polya
     misc_feature    1410..1450
                     /label=BBa_B0012
     terminator      1410..1450
                     /label=BBa_B0012
     promoter        1521..1526
                     /label=Sigma A-35
     promoter        1544..1549
                     /label=Sigma A -10
     promoter        1459..1555
                     /label=BBa_K143012
     RBS             1564..1575
                     /label=rbs
     RBS             1564..1575
                     /label=BBa_K143021
ORIGIN
        1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg
       61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat
      121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc
      181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca
      241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac
      301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa
      361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc
      421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac
      481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag aattttgtca
      541 aaataatttt attgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa
      601 aatgggctcg tgttgtacaa taaatgttac tagagaaagg tggtgaatac tagatggaga
      661 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      721 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      781 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      841 ttgcccgcct gatgaatgct catccggaat ttcgtatggc aatgaaagac ggtgagctgg
      901 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      961 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     1021 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     1081 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     1141 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     1201 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     1261 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taatactaga
     1321 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1381 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1441 tgcgtttata tactagagaa ttttgtcaaa ataattttat tgacaacgtc ttattaacgt
     1501 tgatataatt taaattttat ttgacaaaaa tgggctcgtg ttgtacaata aatgttacta
     1561 gagaaaggtg gtgaa
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