LOCUS       BBa_K143075             1867 bp    DNA     linear       23-May-2024
DEFINITION  B. subtilis AmyE locus GFP output promoter and RBS tester
ACCESSION   BBa_K143075
VERSION     BBa_K143075.1  
FEATURES             Location/Qualifiers
     CDS             1..720
                     /label=GFP protein
     CDS             1..720
                     /label=BBa_E0040
     stem_loop       740..783
                     /label=stem_loop
     misc_feature    729..808
                     /label=BBa_B0010
     terminator      729..808
                     /label=BBa_B0010
     stem_loop       824..843
                     /label=T7 TE
     misc_feature    850..850
                     /label=stop
     polyA_site      844..857
                     /label=polya
     misc_feature    817..857
                     /label=BBa_B0012
     terminator      817..857
                     /label=BBa_B0012
     misc_feature    1..857
                     /label=BBa_I13401
     misc_feature    866..1870
                     /label=3' AmyE homologous sequence
     misc_feature    866..1867
                     /label=BBa_K143002
ORIGIN
        1 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
      721 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      781 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      841 gcctttctgc gtttatatac tagagatccg tttaggctgg gcggtgatag cttctcgttc
      901 aggcagtacg cctcttttct tttccagacc tgagggaggc ggaaatggtg tgaggttccc
      961 ggggaaaagc caaataggcg atcgcgggag tgctttattt gaagatcagg ctatcactgc
     1021 ggtcaataga tttcacaatg tgatggctgg acagcctgag gaactctcga acccgaatgg
     1081 aaacaaccag atatttatga atcagcgcgg ctcacatggc gttgtgctgg caaatgcagg
     1141 ttcatcctct gtctctatca atacggcaac aaaattgcct gatggcaggt atgacaataa
     1201 agctggagcg ggttcatttc aagtgaacga tggtaaactg acaggcacga tcaatgccag
     1261 gtctgtagct gtgctttatc ctgatgatat tgcaaaagcg cctcatgttt tccttgagaa
     1321 ttacaaaaca ggtgtaacac attctttcaa tgatcaactg acgattacct tgcgtgcaga
     1381 tgcgaataca acaaaagccg tttatcaaat caataatgga ccagagacgg cgtttaagga
     1441 tggagatcaa ttcacaatcg gaaaaggaga tccatttggc aaaacataca ccatcatgtt
     1501 aaaaggaacg aacagtgatg gtgtaacgag gaccgagaaa tacagttttg ttaaaagaga
     1561 tccagcgtcg gccaaaacca tcggctatca aaatccgaat cattggagcc aggtaaatgc
     1621 ttatatctat aaacatgatg ggagccgagt aattgaattg accggatctt ggcctggaaa
     1681 accaatgact aaaaatgcag acggaattta cacgctgacg ctgcctgcgg acacggatac
     1741 aaccaacgca aaagtgattt ttaataatgg cagcgcccaa gtgcccggtc agaatcagcc
     1801 tggctttgat tacgtgctaa atggtttata taatgactcg ggcttaagcg gttctcttcc
     1861 ccattga
//