LOCUS BBa_K143078 3366 bp DNA linear 01-Jun-2024 DEFINITION P43-spoVG GFP expression construct ACCESSION BBa_K143078 VERSION BBa_K143078.1 FEATURES Location/Qualifiers misc_feature 1..522 /label=5' AmyE homologous sequence misc_feature 1..522 /label=BBa_K143001 promoter 544..552 /label=Sigma B -35 promoter 568..577 /label=Sigma B -10 promoter 554..559 /label=Sigma A -35 promoter 577..582 /label=Sigma A -10 promoter 531..586 /label=BBa_K143013 RBS 595..606 /label=rbs RBS 595..606 /label=BBa_K143021 misc_feature 613..1272 /label=CmR gene CDS 613..1272 /label=BBa_J31005 stem_loop 1292..1335 /label=stem_loop misc_feature 1281..1360 /label=BBa_B0010 terminator 1281..1360 /label=BBa_B0010 stem_loop 1376..1395 /label=T7 TE misc_feature 1402..1402 /label=stop polyA_site 1396..1409 /label=polya misc_feature 1369..1409 /label=BBa_B0012 terminator 1369..1409 /label=BBa_B0012 promoter 1431..1439 /label=Sigma B -35 promoter 1455..1464 /label=Sigma B -10 promoter 1441..1446 /label=Sigma A -35 promoter 1464..1469 /label=Sigma A -10 promoter 1418..1473 /label=BBa_K143013 RBS 1482..1493 /label=rbs RBS 1482..1493 /label=BBa_K143021 misc_feature 1..1493 /label=BBa_K143072 CDS 1500..2219 /label=GFP protein CDS 1500..2219 /label=BBa_E0040 stem_loop 2239..2282 /label=stem_loop misc_feature 2228..2307 /label=BBa_B0010 terminator 2228..2307 /label=BBa_B0010 stem_loop 2323..2342 /label=T7 TE misc_feature 2349..2349 /label=stop polyA_site 2343..2356 /label=polya misc_feature 2316..2356 /label=BBa_B0012 terminator 2316..2356 /label=BBa_B0012 misc_feature 1500..2356 /label=BBa_I13401 misc_feature 2365..3369 /label=3' AmyE homologous sequence misc_feature 2365..3366 /label=BBa_K143002 misc_feature 1500..3366 /label=BBa_K143075 ORIGIN 1 atgtttgcaa aacgattcaa aacctcttta ctgccgttat tcgctggatt tttattgctg 61 tttcatttgg ttctggcagg accggcggct gcgagtgctg aaacggcgaa caaatcgaat 121 gagcttacag caccgtcgat caaaagcgga accattcttc atgcatggaa ttggtcgttc 181 aatacgttaa aacacaatat gaaggatatt catgatgcag gatatacagc cattcagaca 241 tctccgatta accaagtaaa ggaagggaat caaggagata aaagcatgtc gaactggtac 301 tggctgtatc agccgacatc gtatcaaatt ggcaaccgtt acttaggtac tgaacaagaa 361 tttaaagaaa tgtgtgcagc cgctgaagaa tatggcataa aggtcattgt tgacgcggtc 421 atcaatcata ccaccagtga ttatgccgcg atttccaatg aggttaagag tattccaaac 481 tggacacatg gaaacacaca aattaaaaac tggtctgatc gatactagag attttacatt 541 tttagaaatg ggcgtgaaaa aaagcgcgcg attatgtaaa atataatact agagaaaggt 601 ggtgaatact agatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg 661 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc 721 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat 781 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca 841 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat 901 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt 961 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa 1021 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt 1081 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat 1141 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt 1201 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 1261 ggcggggcgt aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1321 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1381 accttcgggt gggcctttct gcgtttatat actagagatt ttacattttt agaaatgggc 1441 gtgaaaaaaa gcgcgcgatt atgtaaaata taatactaga gaaaggtggt gaatactaga 1501 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg 1561 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa 1621 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg 1681 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc 1741 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca 1801 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta 1861 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat 1921 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa 1981 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc 2041 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc 2101 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc 2161 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat 2221 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 2281 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 2341 cctttctgcg tttatatact agagatccgt ttaggctggg cggtgatagc ttctcgttca 2401 ggcagtacgc ctcttttctt ttccagacct gagggaggcg gaaatggtgt gaggttcccg 2461 gggaaaagcc aaataggcga tcgcgggagt gctttatttg aagatcaggc tatcactgcg 2521 gtcaatagat ttcacaatgt gatggctgga cagcctgagg aactctcgaa cccgaatgga 2581 aacaaccaga tatttatgaa tcagcgcggc tcacatggcg ttgtgctggc aaatgcaggt 2641 tcatcctctg tctctatcaa tacggcaaca aaattgcctg atggcaggta tgacaataaa 2701 gctggagcgg gttcatttca agtgaacgat ggtaaactga caggcacgat caatgccagg 2761 tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc ctcatgtttt ccttgagaat 2821 tacaaaacag gtgtaacaca ttctttcaat gatcaactga cgattacctt gcgtgcagat 2881 gcgaatacaa caaaagccgt ttatcaaatc aataatggac cagagacggc gtttaaggat 2941 ggagatcaat tcacaatcgg aaaaggagat ccatttggca aaacatacac catcatgtta 3001 aaaggaacga acagtgatgg tgtaacgagg accgagaaat acagttttgt taaaagagat 3061 ccagcgtcgg ccaaaaccat cggctatcaa aatccgaatc attggagcca ggtaaatgct 3121 tatatctata aacatgatgg gagccgagta attgaattga ccggatcttg gcctggaaaa 3181 ccaatgacta aaaatgcaga cggaatttac acgctgacgc tgcctgcgga cacggataca 3241 accaacgcaa aagtgatttt taataatggc agcgcccaag tgcccggtca gaatcagcct 3301 ggctttgatt acgtgctaaa tggtttatat aatgactcgg gcttaagcgg ttctcttccc 3361 cattga //