BBa_K1433001

BBa_K1433001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1433001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chaofan Zhang

Date created: 2014-09-27 11:00:00

Date modified: 2015-05-08 01:10:25

attB-J23110-attP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1433001_sequence (Version 1)

Description

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1.
This part is composed of 2 elements.
1. Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.

2. attB and attP sites: Recognition site for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
This part promotes transcription and translation downstream gene, and when treat with Bxb1 gp35, upstream gene will express.

Notes

No

Source

this parts are construct from BBa_J23110 by adding attB/attP sites using PCR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
attB	1,49	feature/dna, sequence_feature
J23110 Promoter	50,84	feature/promoter, promoter
attP	85,137	sequence_feature, feature/dna

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1433001/1

Member of these Collections

• iGEM Parts Registry