LOCUS       BBa_K1439002            1448 bp    DNA     linear       11-Jun-2024
DEFINITION  This part contains a reporter gene BBa_J04450, combined with 
            OriTR. Used to test plasmid mobility.
ACCESSION   BBa_K1439002
VERSION     BBa_K1439002.1  
FEATURES             Location/Qualifiers
     oriT            1..371
                     /label=BBa_J01003
     CDS             380..467
                     /label=end of LacI coding region (inactive)
     misc_feature    380..579
                     /label=BBa_R0010
     misc_feature    468..505
                     /label=CAP binding site
     promoter        516..521
                     /label=-35
     promoter        540..545
                     /label=-10
     misc_feature    545..579
                     /label=LacI binding site
     misc_feature    552..552
                     /label=start
     promoter        380..579
                     /label=BBa_R0010
     misc_feature    592..595
                     /label=conserved
     RBS             588..599
                     /label=BBa_B0034
     CDS             606..1280
                     /label=mrfp1
     misc_feature    1287..1311
                     /label=Help:Barcodes
     CDS             606..1286
                     /label=BBa_E1010
     stem_loop       1331..1374
                     /label=stem_loop
     misc_feature    1320..1399
                     /label=BBa_B0010
     terminator      1320..1399
                     /label=BBa_B0010
     stem_loop       1415..1434
                     /label=T7 TE
     misc_feature    1441..1441
                     /label=stop
     polyA_site      1435..1448
                     /label=polya
     misc_feature    1408..1448
                     /label=BBa_B0012
     terminator      1408..1448
                     /label=BBa_B0012
     misc_feature    380..1448
                     /label=BBa_J04450
ORIGIN
        1 gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt gataggtggg
       61 ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc tgttacgccg
      121 gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag gtgcgaataa
      181 gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat cctgcccggc
      241 tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa tcctgtatat
      301 cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa gcagggttat
      361 gcagcggaaa atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
      421 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
      481 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
      541 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
      601 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
      661 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
      721 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
      781 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
      841 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
      901 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
      961 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
     1021 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
     1081 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
     1141 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
     1201 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
     1261 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
     1321 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1381 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1441 cgtttata
//