BBa_K1444013

BBa_K1444013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1444013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dan Ziemianowicz
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:27

Composite promoter and weak B. subtilis RBS - pTetR



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1444013_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by the repressible promoter pTetR (BBa_R0040), and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pTetR repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1444013/1