BBa_K1444014

BBa_K1444014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1444014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dan Ziemianowicz

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:10:27

Composite promoter and weak B. subtilis RBS - pTetR x2

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1444014_sequence (Version 1)

Description

A composite part consisting of the constitutive promoter Pveg (BBa_K823003), followed by two copies of the repressible promoter pTetR (BBa_R0040), and ending with the weak RBS from B. subtilis (BBa_K780002).

This composite part was intended to control the expression of a downstream reporter, regulated by the expression of the pTetR repressor protein. The constitutive promoter Pveg allows reliable expression when the repressible promoter is not repressed. The duplicated repressible promoter allows for stronger repression.

Notes

No interference with endogenous cell signalling.

Source

B. subtilis

Components

Access	Instance	Definition
public	BBa_K823003	BBa_K823003
public	BBa_R0040	p(tetR)
public	BBa_K090505	BBa_K090505

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K823003	1,237	BBa_K823003
BBa_R0040	246,299	p(tetR)
BBa_K090505	308,318	BBa_K090505

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1444014/1

Member of these Collections

• iGEM Parts Registry