LOCUS       BBa_K145000             1033 bp    DNA     linear       13-May-2024
DEFINITION  eCFP with LVA tag under OmpR control
ACCESSION   BBa_K145000
VERSION     BBa_K145000.1  
FEATURES             Location/Qualifiers
     misc_feature    11..28
                     /label=F1 OmpR
     misc_feature    31..48
                     /label=F2 OmpR
     misc_feature    51..68
                     /label=F3 OmpR
     promoter        73..78
                     /label=-35
     promoter        88..93
                     /label=-10
     promoter        1..108
                     /label=BBa_R0084
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..896
                     /label=CFP (LVA)
     misc_feature    853..890
                     /label=SsrA
     misc_feature    891..896
                     /label=2
     misc_feature    203..203
                     /label=A
     misc_feature    135..896
                     /label=BBa_E0022
     misc_feature    135..896
                     /label=BBa_E0022
     stem_loop       916..959
                     /label=stem_loop
     misc_feature    905..984
                     /label=BBa_B0010
     terminator      905..984
                     /label=BBa_B0010
     stem_loop       1000..1019
                     /label=T7 TE
     misc_feature    1026..1026
                     /label=stop
     polyA_site      1020..1033
                     /label=polya
     misc_feature    993..1033
                     /label=BBa_B0012
     terminator      993..1033
                     /label=BBa_B0012
ORIGIN
        1 cttaaatttt acttttggtt acatattttt tctttttgaa accaaatctt tatctttgta
       61 gcactttcac ggtagcgaaa cgttagtttg aatggaaaga tgcctgcata ctagagaaag
      121 aggagaaata ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      181 tggtcgagct ggacggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg
      241 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      301 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
      361 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      421 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
      481 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
      541 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
      601 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
      661 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
      721 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
      781 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
      841 agctgtacaa gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact
      901 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      961 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1021 ttctgcgttt ata
//