LOCUS       BBa_K145002             2926 bp    DNA     linear       13-May-2024
DEFINITION  T7 RNA polymerase with UmuD derived tag under OmpF control
ACCESSION   BBa_K145002
VERSION     BBa_K145002.1  
FEATURES             Location/Qualifiers
     misc_feature    11..28
                     /label=F1 OmpR
     misc_feature    31..48
                     /label=F2 OmpR
     misc_feature    51..68
                     /label=F3 OmpR
     promoter        73..78
                     /label=-35
     promoter        88..93
                     /label=-10
     promoter        1..108
                     /label=BBa_R0084
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     misc_feature    135..137
                     /label=start
     misc_feature    2784..2786
                     /label=stop
     misc_feature    2787..2789
                     /label=stop
     CDS             138..2783
                     /label=protein
     CDS             135..2789
                     /label=BBa_K145001
     stem_loop       2809..2852
                     /label=stem_loop
     misc_feature    2798..2877
                     /label=BBa_B0010
     terminator      2798..2877
                     /label=BBa_B0010
     stem_loop       2893..2912
                     /label=T7 TE
     misc_feature    2919..2919
                     /label=stop
     polyA_site      2913..2926
                     /label=polya
     misc_feature    2886..2926
                     /label=BBa_B0012
     terminator      2886..2926
                     /label=BBa_B0012
     terminator      2798..2926
                     /label=BBa_B0015
ORIGIN
        1 cttaaatttt acttttggtt acatattttt tctttttgaa accaaatctt tatctttgta
       61 gcactttcac ggtagcgaaa cgttagtttg aatggaaaga tgcctgcata ctagagaaag
      121 aggagaaata ctagatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac
      181 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac
      241 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg
      301 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta
      361 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc
      421 gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt
      481 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg
      541 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg
      601 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc
      661 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac
      721 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct
      781 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg
      841 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa
      901 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta
      961 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc
     1021 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg
     1081 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag
     1141 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg
     1201 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg
     1261 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta
     1321 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt
     1381 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag
     1441 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag
     1501 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc
     1561 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc
     1621 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct
     1681 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt
     1741 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg
     1801 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta
     1861 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta
     1921 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac
     1981 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga
     2041 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc
     2101 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat
     2161 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa
     2221 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg
     2281 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt
     2341 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc
     2401 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt
     2461 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag
     2521 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta
     2581 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat
     2641 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt
     2701 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct
     2761 tagagtcgga cttcgcgttc gcgtaataat actagagcca ggcatcaaat aaaacgaaag
     2821 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     2881 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//