LOCUS       BBa_K145103             2971 bp    DNA     linear       18-May-2024
DEFINITION  T7 with UmuD derived tag under constitutive promotor + RiboLock3d
ACCESSION   BBa_K145103
VERSION     BBa_K145103.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..86
                     /label=BBa_J23032
     misc_feature    93..95
                     /label=start
     misc_feature    2832..2834
                     /label=stop
     misc_feature    2829..2831
                     /label=stop
     misc_feature    93..179
                     /label=tag
     CDS             180..2828
                     /label=cds
     CDS             93..2834
                     /label=BBa_K145014
     stem_loop       2854..2897
                     /label=stem_loop
     misc_feature    2843..2922
                     /label=BBa_B0010
     terminator      2843..2922
                     /label=BBa_B0010
     stem_loop       2938..2957
                     /label=T7 TE
     misc_feature    2964..2964
                     /label=stop
     polyA_site      2958..2971
                     /label=polya
     misc_feature    2931..2971
                     /label=BBa_B0012
     terminator      2931..2971
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaactaga atcacctctt
       61 gcttttgggt aagacagaag aggagatact agatgttgtt tatcaagcct gcggatctcc
      121 gcgaaattgt gacttttccg ctatttagcg atcttgttca gtgtggcttt ccttcaccga
      181 tgaacacgat taacatcgct aagaacgact tctctgacat cgaactggct gctatcccgt
      241 tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg gcccttgagc
      301 atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt caacttaaag
      361 ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta ctccctaaga
      421 tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc aagcgcccga
      481 cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc accattaaga
      541 ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta gcaagcgcaa
      601 tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt gaagctaagc
      661 acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc tacaagaaag
      721 catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt ggcgaggcgt
      781 ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc gagatgctca
      841 ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta ggtcaagact
      901 ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt gcaggtgcgc
      961 tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg tggactggca
     1021 ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg cgtactcaca
     1081 gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac aaagcgatta
     1141 acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc gccaacgtaa
     1201 tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt gaagaactcc
     1261 cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg aaacgtgctg
     1321 ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc cttgagttca
     1381 tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct tacaacatgg
     1441 actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac gatatgacca
     1501 aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac tactggctga
     1561 aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag cgcatcaagt
     1621 tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg gagaacactt
     1681 ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag tacgctgggg
     1741 tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac gggtcttgct
     1801 ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc gcggttaact
     1861 tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa gtcaacgaga
     1921 ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg accgatgaga
     1981 acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct ggtcaatggc
     2041 tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg gcttacgggt
     2101 ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca gctattgatt
     2161 ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg gctaagctga
     2221 tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac tggcttaagt
     2281 ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag attcttcgca
     2341 agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag gaatacaaga
     2401 agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta cagcctacca
     2461 ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt atcgctccta
     2521 actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg gcacacgaga
     2581 agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt ccggctgacg
     2641 ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag tcttgtgatg
     2701 tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa ttggacaaaa
     2761 tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag tcggacttcg
     2821 cgttcgcgta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     2881 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     2941 tcaccttcgg gtgggccttt ctgcgtttat a
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