LOCUS       BBa_K145106             1060 bp    DNA     linear       04-May-2024
DEFINITION  cI P<sub>RM</sub> PoPS -> Lactonase
ACCESSION   BBa_K145106
VERSION     BBa_K145106.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     RBS             91..103
                     /label=RBS-3\Weak
     RBS             97..100
                     /label=RBS
     misc_feature    91..103
                     /label=BBa_B0032
     RBS             91..103
                     /label=BBa_B0032
     misc_feature    110..112
                     /label=start
     misc_feature    893..898
                     /label=2
     misc_feature    860..892
                     /label=LVA
     CDS             110..859
                     /label=aiiA
     misc_feature    110..898
                     /label=BBa_C0060
     misc_feature    899..923
                     /label=Help:Barcodes
     CDS             110..898
                     /label=BBa_C0060
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     terminator      932..1060
                     /label=BBa_B0015
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag tcacacagga aagtactaga tgacagtaaa
      121 gaagctttat ttcgtcccag caggtcgttg tatgttggat cattcgtctg ttaatagtac
      181 attaacacca ggagaattat tagacttacc ggtttggtgt tatcttttgg agactgaaga
      241 aggacctatt ttagtagata caggtatgcc agaaagtgca gttaataatg aaggtctttt
      301 taacggtaca tttgtcgaag ggcaggtttt accgaaaatg actgaagaag atagaatcgt
      361 gaatatttta aaacgggttg gttatgagcc ggaagacctt ctttatatta ttagttctca
      421 cttgcatttt gatcatgcag gaggaaatgg cgcttttata aatacaccaa tcattgtaca
      481 gcgtgctgaa tatgaggcgg cgcagcatag cgaagaatat ttgaaagaat gtatattgcc
      541 gaatttaaac tacaaaatca ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt
      601 gcatacacca ggccatactc cagggcatca atcgctatta attgagacag aaaaatccgg
      661 tcctgtatta ttaacgattg atgcatcgta tacgaaagag aattttgaaa atgaagtgcc
      721 atttgcggga tttgattcag aattagcttt atcttcaatt aaacgtttaa aagaagtggt
      781 gatgaaagag aagccgattg ttttctttgg acatgatata gagcaggaaa ggggatgtaa
      841 agtgttccct gaatatatag ctgcaaacga cgaaaactac gctttagtag cttaataacg
      901 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata
//