LOCUS       BBa_K145107             2156 bp    DNA     linear       20-May-2024
DEFINITION  Lactonase pulse generator
ACCESSION   BBa_K145107
VERSION     BBa_K145107.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     promoter        92..97
                     /label=-10
     promoter        69..74
                     /label=-35
     misc_feature    79..95
                     /label=OR1
     misc_feature    55..71
                     /label=OR2
     misc_feature    55..103
                     /label=BBa_R0051
     promoter        55..103
                     /label=BBa_R0051
     misc_feature    112..154
                     /label=BBa_J23032
     misc_feature    169..172
                     /label=conserved
     RBS             163..176
                     /label=BBa_B0031
     misc_feature    921..926
                     /label=2
     CDS             183..926
                     /label=cI HK022
     misc_feature    888..920
                     /label=SsrA
     misc_feature    183..190
                     /label=OR3 partial
     misc_feature    183..926
                     /label=BBa_C0050
     misc_feature    927..951
                     /label=Help:Barcodes
     CDS             183..926
                     /label=BBa_C0050
     stem_loop       971..1014
                     /label=stem_loop
     misc_feature    960..1039
                     /label=BBa_B0010
     terminator      960..1039
                     /label=BBa_B0010
     stem_loop       1055..1074
                     /label=T7 TE
     misc_feature    1081..1081
                     /label=stop
     polyA_site      1075..1088
                     /label=polya
     misc_feature    1048..1088
                     /label=BBa_B0012
     terminator      1048..1088
                     /label=BBa_B0012
     misc_feature    163..1088
                     /label=BBa_P0150
     misc_feature    1..1088
                     /label=BBa_K145105
     misc_feature    1105..1121
                     /label=OR1
     misc_feature    1129..1145
                     /label=OR2
     promoter        1144..1149
                     /label=-35
     misc_feature    1155..1161
                     /label=mutate to obliterate OR3
     promoter        1167..1172
                     /label=-10
     promoter        1097..1178
                     /label=BBa_I12007
     RBS             1187..1199
                     /label=RBS-3\Weak
     RBS             1193..1196
                     /label=RBS
     misc_feature    1187..1199
                     /label=BBa_B0032
     RBS             1187..1199
                     /label=BBa_B0032
     misc_feature    1206..1208
                     /label=start
     misc_feature    1989..1994
                     /label=2
     misc_feature    1956..1988
                     /label=LVA
     CDS             1206..1955
                     /label=aiiA
     misc_feature    1206..1994
                     /label=BBa_C0060
     misc_feature    1995..2019
                     /label=Help:Barcodes
     CDS             1206..1994
                     /label=BBa_C0060
     stem_loop       2039..2082
                     /label=stem_loop
     misc_feature    2028..2107
                     /label=BBa_B0010
     terminator      2028..2107
                     /label=BBa_B0010
     stem_loop       2123..2142
                     /label=T7 TE
     misc_feature    2149..2149
                     /label=stop
     polyA_site      2143..2156
                     /label=polya
     misc_feature    2116..2156
                     /label=BBa_B0012
     terminator      2116..2156
                     /label=BBa_B0012
     terminator      2028..2156
                     /label=BBa_B0015
     misc_feature    1097..2156
                     /label=BBa_K145106
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagtaacac
       61 cgtgcgtgtt gactatttta cctctggcgg tgataatggt tgctactaga gaactagaat
      121 cacctcttgc ttttgggtaa gacagaagag gagatactag agtcacacag gaaacctact
      181 agatggttca acagaaagag cgtgaaactt tctcgcagag gcttgcgctg gcctgtgata
      241 aagcgggatt acctttgcat ggtaggcagg ctgatttagc tgtcaggctt aaggtcacac
      301 caaaagccat tagtaaatgg ttcaacgggg agtcaatacc aagaaaagac aagatggaat
      361 ctctggcttc ggtgctggga actactgctg catatctgca tggctatgct gatgatgacg
      421 gtatcacggt aaatcatcta tcaagatcaa atgattatta tcgtgttgat gtattggatg
      481 ttcaggcgag cgccgggcca ggaaccatgg tttccaatga atttatagaa aagataagag
      541 caattgaata tacgaccgag caggcaagaa ttttatttaa tggaaggcca caggaaagcg
      601 taaaagtcat cacggttcgc ggtgacagca tggagggaac catcaatccg ggagatgaga
      661 tctttgttga tgtatccata acctgttttg atggcgatgg catttatgtg tttgtatacg
      721 ggaaaacaat gcacgttaag cgcctgcaaa tgcaaaagaa caggcttgcc gtcatctctg
      781 acaatgccgc ttatgatcga tggtacatag aagaaggtga agaagagcaa cttcacattc
      841 tagccaaagt cctcattagg cagtcaatcg attacaagcg attcggagct gcaaacgacg
      901 aaaactacgc tttagtagct taataaccct gatagtgcta gtgtagatcc ctactagagc
      961 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1021 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1081 cgtttatata ctagaggcaa ccattatcac cgccagaggt aaaatagtca acacgcacgg
     1141 tgttagatat ttataaatag tggtgataga tttaacgtta ctagagtcac acaggaaagt
     1201 actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg ttggatcatt
     1261 cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt tggtgttatc
     1321 ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa agtgcagtta
     1381 ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg aaaatgactg
     1441 aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa gaccttcttt
     1501 atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct tttataaata
     1561 caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa gaatatttga
     1621 aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat gaagtcgtac
     1681 caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg ctattaattg
     1741 agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg aaagagaatt
     1801 ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct tcaattaaac
     1861 gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat gatatagagc
     1921 aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa aactacgctt
     1981 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat
     2041 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2101 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//