BBa_R0084
1
OmpR
Promoter (OmpR, positive)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
NC_000913 E. coli K12
Released HQ 2013
Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
false
false
_1_
0
24
7
In stock
false
The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
true
Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
annotation301313
1
F3 OmpR
range301313
1
51
68
annotation301309
1
F2 OmpR
range301309
1
31
48
annotation301317
1
-35
range301317
1
73
78
annotation301318
1
-10
range301318
1
88
93
annotation301308
1
F1 OmpR
range301308
1
11
28
BBa_R0053
1
cII p22
Promoter (p22 cII regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Bacteriophage p22.
Released HQ 2013
The p22 cII regulatory region sequence is a 97 base-pair sequence with the standard BioBrick prefix and suffix sections on its ends. p22 cII repressor protein, BBa_C0053, binds to it.<br> This segment contains O-R1, O-R2, a fragment of O-R3, the -35 of P-RM, and P-R (-10 and -35 from Tom Knight)</p>
false
false
_1_
0
24
7
In stock
false
<P> <P><P>
true
Maia Mahoney
annotation2041
1
-35
range2041
1
8
13
annotation2035
1
OR3
range2035
1
1
3
annotation7069
1
BBa_R0053
range7069
1
1
54
annotation2042
1
-10
range2042
1
30
35
annotation2038
1
-35
range2038
1
18
23
annotation2037
1
OR1
range2037
1
34
51
annotation2036
1
OR2
range2036
1
11
28
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K145013
1
asLuxI
antisense LuxI
2008-07-09T11:00:00Z
2015-05-08T01:10:28Z
BBa_C0061
antisense LuxI
false
false
_257_
0
2970
9
In stock
true
just antisense
true
Jonas Demeulemeester
BBa_K145111
1
BBa_K145111
OmpF PoPS -> deactivate constitutive antisense LuxI
2008-07-09T11:00:00Z
2015-05-08T01:10:28Z
other parts
Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor
false
false
_257_
0
2970
9
Not in stock
false
validate dual promotor system!
false
Jonas Demeulemeester
component2234217
1
BBa_K145013
component2234185
1
BBa_I746350
component2234192
1
BBa_B0015
component2234194
1
BBa_I746364
component2234199
1
BBa_C0053
component2234196
1
BBa_B0032
component2234179
1
BBa_R0084
component2234181
1
BBa_I746364
component2234211
1
BBa_R0053
component2234224
1
BBa_B0015
component2234209
1
BBa_B0015
annotation2234224
1
BBa_B0015
range2234224
1
2265
2393
annotation2234181
1
BBa_I746364
range2234181
1
117
209
annotation2234194
1
BBa_I746364
range2234194
1
600
692
annotation2234199
1
BBa_C0053
range2234199
1
720
1406
annotation2234196
1
BBa_B0032
range2234196
1
701
713
annotation2234209
1
BBa_B0015
range2234209
1
1440
1568
annotation2234211
1
BBa_R0053
range2234211
1
1577
1630
annotation2234192
1
BBa_B0015
range2234192
1
463
591
annotation2234217
1
BBa_K145013
range2234217
1
1639
2256
annotation2234185
1
BBa_I746350
range2234185
1
218
454
annotation2234179
1
BBa_R0084
range2234179
1
1
108
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_I746350
1
BBa_I746350
ogr activator from P2 phage
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
plasmid DNA supplied by Prof. Richard Calendar, University of California.
The ogr activator taken from P2 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees.
The part sequence does already contain a ribosome binding site (B0034)!
false
false
_116_
0
2122
9
In stock
true
The part does contain a RBS (B0034) already.
true
Stefan Milde
annotation1943865
1
B0034
range1943865
1
1
12
annotation1943866
1
P2 ogr
range1943866
1
19
19
annotation1943867
1
P2 ogr
range1943867
1
19
237
BBa_I746364
1
BBa_I746364
Psid promoter from P4 phage
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
P4 phage genome
Released HQ 2013
This is the Psid promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the Psid promoter.
false
false
_116_
0
2122
9
In stock
false
no special considerations
true
Stefan Milde
annotation1943787
1
Psid
range1943787
1
1
93
BBa_C0053
1
c2 P22
c2 repressor from Salmonella phage P22 (+LVA)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
Bacteriophage P22
Released HQ 2013
The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.</p>
false
false
_1_
0
24
7
In stock
false
References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P> References (unparsed) here: <p>Vander Byl,C. and Kropinski,A.M. <em>Sequence of the genome of Salmonella bacteriophage P22</em><br> J. Bacteriol. 182 (22), 6472-6481 (2000) <P><P>
true
Maia Mahoney
annotation1751
1
stop
range1751
1
682
687
annotation2213993
1
Help:Barcodes
range2213993
1
688
712
annotation1747
1
cII p22
range1747
1
1
648
annotation7036
1
BBa_C0053
range7036
1
1
687
annotation1750
1
LVA
range1750
1
649
681
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0053_sequence
1
aataaacttgactaaagattcctttagtagataatttaagtgttctttaatttc
BBa_K145111_sequence
1
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgcatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttcttatactagagtcacacaggaaagtactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaataaacttgactaaagattcctttagtagataatttaagtgttctttaatttctactagagttattaagctactaaagcgtagttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcatagacaatacaaccgatttagtatcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgctttaaaaatcgctctattgctgttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagcttcaaatagtttcattgtaatttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaattcgactatattaggatctttgggagcactctgttgaccaagcaattcaggaaaaacacttttcagcatataatcacctgttgtaggtaataaacgccagcatccacttacattttcagtatcatcacaagcataaatatattctgcatttgagttatcatactcatctgattcaaggttattttctacaactaagtcccactcaagtctttgcttaaacacttgataacgaagacttagaatacctttatactcctccgatggaattgccaaaaaatccgatttttttatcattatagtcattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I746350_sequence
1
aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa
BBa_K145013_sequence
1
ttattaagctactaaagcgtagttttcgtcgtttgcagcatttaagactgcttttttaaactgttcattaataggcatagacaatacaaccgatttagtatcacctaatacatgaatttctttgtctccaatacgatgacaaggaactttaatacgctttaaaaatcgctctattgctgttgatgttactgttacatattctgtaataccttgactaacagcgtgtttatatatagcttcaaatagtttcattgtaatttcactagcagagttatttatctttgagctatttttacctacagcaaaacgacttaattcgactatattaggatctttgggagcactctgttgaccaagcaattcaggaaaaacacttttcagcatataatcacctgttgtaggtaataaacgccagcatccacttacattttcagtatcatcacaagcataaatatattctgcatttgagttatcatactcatctgattcaaggttattttctacaactaagtcccactcaagtctttgcttaaacacttgataacgaagacttagaatacctttatactcctccgatggaattgccaaaaaatccgatttttttatcattatagtcat
BBa_B0032_sequence
1
tcacacaggaaag
BBa_I746364_sequence
1
tggcttgccggtctgaggatgagtctcctgtgtcagggctggcacatctgcaatgcgtcgtgttgttgtccggtgtacgtcacaattttctta
BBa_R0084_sequence
1
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca
BBa_C0053_sequence
1
atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z