LOCUS BBa_K145210 2701 bp DNA linear 18-May-2024 DEFINITION MEMORY ACCESSION BBa_K145210 VERSION BBa_K145210.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..73 /label=RBS-4\Weaker RBS 69..72 /label=RBS misc_feature 63..73 /label=BBa_B0033 RBS 63..73 /label=BBa_B0033 misc_feature 710..715 /label=2 CDS 80..715 /label=cI 434 CDS 80..715 /label=BBa_C0056 stem_loop 735..778 /label=stem_loop misc_feature 724..803 /label=BBa_B0010 terminator 724..803 /label=BBa_B0010 stem_loop 819..838 /label=T7 TE misc_feature 845..845 /label=stop polyA_site 839..852 /label=polya misc_feature 812..852 /label=BBa_B0012 terminator 812..852 /label=BBa_B0012 terminator 724..852 /label=BBa_B0015 misc_feature 861..863 /label=OR3 misc_feature 871..888 /label=OR2 misc_feature 894..911 /label=OR1 promoter 878..883 /label=-35 promoter 868..873 /label=-35 promoter 890..895 /label=-10 misc_feature 861..914 /label=BBa_R0053 promoter 861..914 /label=BBa_R0053 misc_feature 929..932 /label=conserved RBS 923..936 /label=BBa_B0031 CDS 943..1611 /label=cI 434 misc_feature 1573..1611 /label=LVA misc_feature 943..1611 /label=BBa_C0052 misc_feature 1612..1636 /label=Help:Barcodes CDS 943..1611 /label=BBa_C0052 stem_loop 1656..1699 /label=stem_loop misc_feature 1645..1724 /label=BBa_B0010 terminator 1645..1724 /label=BBa_B0010 stem_loop 1740..1759 /label=T7 TE misc_feature 1766..1766 /label=stop polyA_site 1760..1773 /label=polya misc_feature 1733..1773 /label=BBa_B0012 terminator 1733..1773 /label=BBa_B0012 misc_feature 923..1773 /label=BBa_P0152 promoter 1782..1824 /label=-10 misc_feature 1789..1802 /label=OR2 misc_feature 1811..1824 /label=OR1 promoter 1782..1787 /label=-35 promoter 1783..1788 /label=-35 misc_feature 1816..1818 /label=start promoter 1805..1810 /label=-10 misc_feature 1782..1827 /label=BBa_R1052 promoter 1782..1827 /label=BBa_R1052 RBS 1836..1846 /label=RBS-4\Weaker RBS 1842..1845 /label=RBS misc_feature 1836..1846 /label=BBa_B0033 RBS 1836..1846 /label=BBa_B0033 CDS 1853..2500 /label=cII p22 misc_feature 2501..2533 /label=LVA misc_feature 2534..2539 /label=stop misc_feature 1853..2539 /label=BBa_C0053 misc_feature 2540..2564 /label=Help:Barcodes CDS 1853..2539 /label=BBa_C0053 stem_loop 2584..2627 /label=stem_loop misc_feature 2573..2652 /label=BBa_B0010 terminator 2573..2652 /label=BBa_B0010 stem_loop 2668..2687 /label=T7 TE misc_feature 2694..2694 /label=stop polyA_site 2688..2701 /label=polya misc_feature 2661..2701 /label=BBa_B0012 terminator 2661..2701 /label=BBa_B0012 misc_feature 1836..2701 /label=BBa_P0353 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agtcacacag gactactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct 121 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca 181 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg 241 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca 301 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga 361 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt 421 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca 481 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag 541 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga 601 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg 661 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtat aataatacta 721 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 781 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 841 tctgcgttta tatactagag aataaacttg actaaagatt cctttagtag ataatttaag 901 tgttctttaa tttctactag agtcacacag gaaacctact agatgagtat ttcttccagg 961 gtaaaaagca aaagaatcca gcttggactt aaccaggctg aacttgctca aaaggtgggg 1021 actacccagc agtctataga gcagctcgaa aacggtaaaa ctaagcgacc acgcttttta 1081 ccagaacttg cgtcagctct tggcgtaagt gttgactggc tgctcaatgg cacctctgat 1141 tcgaatgtta gatttgttgg gcacgttgag cccaaaggga aatatccatt gattagcatg 1201 gttagagctg gttcgtggtg tgaagcttgt gaaccctacg atatcaagga cattgatgaa 1261 tggtatgaca gtgacgttaa cttattaggc aatggattct ggctgaaggt tgaaggtgat 1321 tccatgacct cacctgtagg tcaaagcatc cctgaaggtc atatggtgtt agtagatact 1381 ggacgggagc cagtgaatgg aagccttgtt gtagccaaac tgactgacgc gaacgaagca 1441 acattcaaga aactggtcat agatggcggt cagaagtacc tgaaaggcct gaatccttca 1501 tggcctatga ctcctatcaa cggaaactgc aagattatcg gtgttgtcgt ggaagcgagg 1561 gtaaaattcg tagctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag 1621 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag 1681 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg 1741 gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacaaac aagatacatt 1801 gtatgatact acaagaaagt ttgttgatac tagagtcaca caggactact agatgaatac 1861 acaattgatg ggtgagcgta ttcgcgctcg aagaaaaaaa ctcaagatta gacaagccgc 1921 tcttggtaag atggtgggag tgtctaatgt tgcaatatcg caatgggagc gctcggagac 1981 tgagccaaat ggggagaacc tgttggcact ttcgaaggct cttcagtgct cccctgacta 2041 tttgctgaaa ggagatttaa gccagacaaa cgttgcctat catagtaggc atgagccaag 2101 aggatcatac cctcttatca gttgggtaag cgcagggcaa tggatggaag ctgtagaacc 2161 ttatcacaag cgcgcgatag agaactggca cgacaccact gtagattgtt cagaagattc 2221 attttggctt gatgtccaag gtgactctat gacagcaccg gcagggttaa gcattccaga 2281 aggaatgata attctggttg atcccgaagt cgaaccaaga aacggcaagc tggttgttgc 2341 aaaattagaa ggtgaaaacg aggccacatt caaaaaatta gttatggatg caggccgaaa 2401 gtttttaaaa ccattaaacc cacaatatcc gatgatagaa atcaacggaa actgcaaaat 2461 cattggcgta gttgttgacg caaaactcgc aaatcttcca gctgcaaacg acgaaaacta 2521 cgctttagta gcttaataac actgatagtg ctagtgtaga tcactactag agccaggcat 2581 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2641 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2701 a //