LOCUS       BBa_K145258              852 bp    DNA     linear       23-May-2024
DEFINITION  MEMORY Part 1
ACCESSION   BBa_K145258
VERSION     BBa_K145258.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..73
                     /label=RBS-4\Weaker
     RBS             69..72
                     /label=RBS
     misc_feature    63..73
                     /label=BBa_B0033
     RBS             63..73
                     /label=BBa_B0033
     misc_feature    710..715
                     /label=2
     CDS             80..715
                     /label=cI 434
     CDS             80..715
                     /label=BBa_C0056
     stem_loop       735..778
                     /label=stem_loop
     misc_feature    724..803
                     /label=BBa_B0010
     terminator      724..803
                     /label=BBa_B0010
     stem_loop       819..838
                     /label=T7 TE
     misc_feature    845..845
                     /label=stop
     polyA_site      839..852
                     /label=polya
     misc_feature    812..852
                     /label=BBa_B0012
     terminator      812..852
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gactactaga tgagtatttc ttccagggta aaaagcaaaa gaatccagct
      121 tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt ctatagagca
      181 gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt cagctcttgg
      241 cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat ttgttgggca
      301 cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt cgtggtgtga
      361 agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg acgttaactt
      421 attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac ctgtaggtca
      481 aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag tgaatggaag
      541 ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac tggtcataga
      601 tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc ctatcaacgg
      661 aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtat aataatacta
      721 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      781 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      841 tctgcgttta ta
//