LOCUS       BBa_K145268             1819 bp    DNA     linear       01-Jun-2024
DEFINITION  C2 P22 generator controlled by tetracyclin
ACCESSION   BBa_K145268
VERSION     BBa_K145268.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    1..883
                     /label=BBa_K145201
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     RBS             954..964
                     /label=RBS-4\Weaker
     RBS             960..963
                     /label=RBS
     misc_feature    954..964
                     /label=BBa_B0033
     RBS             954..964
                     /label=BBa_B0033
     CDS             971..1618
                     /label=cII p22
     misc_feature    1619..1651
                     /label=LVA
     misc_feature    1652..1657
                     /label=stop
     misc_feature    971..1657
                     /label=BBa_C0053
     misc_feature    1658..1682
                     /label=Help:Barcodes
     CDS             971..1657
                     /label=BBa_C0053
     stem_loop       1702..1745
                     /label=stem_loop
     misc_feature    1691..1770
                     /label=BBa_B0010
     terminator      1691..1770
                     /label=BBa_B0010
     stem_loop       1786..1805
                     /label=T7 TE
     misc_feature    1812..1812
                     /label=stop
     polyA_site      1806..1819
                     /label=polya
     misc_feature    1779..1819
                     /label=BBa_B0012
     terminator      1779..1819
                     /label=BBa_B0012
     misc_feature    954..1819
                     /label=BBa_P0353
     misc_feature    892..1819
                     /label=BBa_K145273
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca
      961 ggactactag atgaatacac aattgatggg tgagcgtatt cgcgctcgaa gaaaaaaact
     1021 caagattaga caagccgctc ttggtaagat ggtgggagtg tctaatgttg caatatcgca
     1081 atgggagcgc tcggagactg agccaaatgg ggagaacctg ttggcacttt cgaaggctct
     1141 tcagtgctcc cctgactatt tgctgaaagg agatttaagc cagacaaacg ttgcctatca
     1201 tagtaggcat gagccaagag gatcataccc tcttatcagt tgggtaagcg cagggcaatg
     1261 gatggaagct gtagaacctt atcacaagcg cgcgatagag aactggcacg acaccactgt
     1321 agattgttca gaagattcat tttggcttga tgtccaaggt gactctatga cagcaccggc
     1381 agggttaagc attccagaag gaatgataat tctggttgat cccgaagtcg aaccaagaaa
     1441 cggcaagctg gttgttgcaa aattagaagg tgaaaacgag gccacattca aaaaattagt
     1501 tatggatgca ggccgaaagt ttttaaaacc attaaaccca caatatccga tgatagaaat
     1561 caacggaaac tgcaaaatca ttggcgtagt tgttgacgca aaactcgcaa atcttccagc
     1621 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
     1681 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1741 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1801 gggcctttct gcgtttata
//