LOCUS       BBa_K145280             1868 bp    DNA     linear       17-May-2024
DEFINITION  GFP-LVA under TetR control
ACCESSION   BBa_K145280
VERSION     BBa_K145280.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     terminator      755..883
                     /label=BBa_B0015
     misc_feature    1..883
                     /label=BBa_K145201
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     RBS             954..966
                     /label=RBS-3\Weak
     RBS             960..963
                     /label=RBS
     misc_feature    954..966
                     /label=BBa_B0032
     RBS             954..966
                     /label=BBa_B0032
     misc_feature    973..975
                     /label=start
     misc_feature    1726..1728
                     /label=stop
     misc_feature    1729..1731
                     /label=stop
     misc_feature    1689..1725
                     /label=LVA tag
     CDS             976..1688
                     /label=cds
     misc_feature    973..1731
                     /label=BBa_K145015
     stem_loop       1751..1794
                     /label=stem_loop
     misc_feature    1740..1819
                     /label=BBa_B0010
     terminator      1740..1819
                     /label=BBa_B0010
     stem_loop       1835..1854
                     /label=T7 TE
     misc_feature    1861..1861
                     /label=stop
     polyA_site      1855..1868
                     /label=polya
     misc_feature    1828..1868
                     /label=BBa_B0012
     terminator      1828..1868
                     /label=BBa_B0012
     misc_feature    892..1868
                     /label=BBa_K145205
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagtcacaca
      961 ggaaagtact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     1021 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     1081 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     1141 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1201 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1261 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1321 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1381 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1441 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1501 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1561 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1621 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1681 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc
     1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1861 cgtttata
//