BBa_K1453000 1 BBa_K1453000 ssDsbA-mRFP-Lgt-TAL USB-His Tag 2014-09-30T11:00:00Z 2015-05-08T01:10:29Z BBa_K771000 BBa_E1010 This part is designed as a scaffold of connectee. The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 The TAL adapter part contains T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI, in order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), which is designed by iGEM12_Freiburg. false false _1831_ 0 21354 9 It's complicated false When digest with BsmBI, this part can create two sticky-ends, one can match with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, the other can match with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`. With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), user can synthesize a 14 nucleotide Transactivator-like (TAL) protein to suit for their own connector and design their own polymerization. (Golden Gate Cloning) More details about membrane anchor, please find http://2012.igem.org/Team:SJTU-BioX-Shanghai. More details about TAL and Golden Gate Cloning, please find http://2012.igem.org/Team:Freiburg. false Renhe Luo annotation2391452 1 mRFP range2391452 1 58 732 annotation2391457 1 His TAG range2391457 1 2143 2160 annotation2391450 1 BBa_K1453000 range2391450 1 1 2163 annotation2391474 1 ATG range2391474 1 1 3 annotation2391455 1 FL range2391455 1 1654 1710 annotation2391458 1 TAA range2391458 1 2161 2163 annotation2391453 1 HL range2391453 1 733 780 annotation2391454 1 Lgt range2391454 1 781 1653 annotation2391451 1 ssDsbA range2391451 1 1 57 annotation2391456 1 TAL USB range2391456 1 1711 2142 BBa_K1453000_sequence 1 atgaaaaagatttggctggcgctggctggtttagttttagcgtttagcgcatcggcgatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctgctgaggccgccgcaaaagaagcagcagctaaggaagctgcggcgaagatgaccagtagctatctgcattttccggagtttgatccggtcattttctcaataggacccgtggcgcttcactggtacggcctgatgtatctggtgggtttcatttttgcaatgtggctggcaacacgacgggcgaatcgtccgggcagcggctggaccaaaaatgaagttgaaaacttactctatgcgggcttcctcggcgtcttcctcgggggacgtattggttatgttctgttctacaatttcccgcagtttatggccgatccgctgtatctgttccgtgtctgggacggcggcatgtctttccacggcggcctgattggcgttatcgtggtgatgattatcttcgcccgccgtactaaacgttccttcttccaggtctctgattttatcgcaccactcattccgtttggtcttggtgccgggcgtctgggcaactttattaacggtgaattgtggggccgcgttgacccgaacttcccgtttgccatgctgttccctggctcccgtacagaagatattttgctgctgcaaaccaacccgcagtggcaatccattttcgacacttacggtgtgctgccgcgccacccatcacagctttacgagctgctgctggaaggtgtggtgctgtttattatcctcaacctgtatattcgtaaaccacgcccaatgggagctgtctcaggtttgttcctgattggttacggcgcgtttcgcatcattgttgagtttttccgccagcccgacgcgcagtttaccggtgcccgggtgcagtacatcagcatggggcaaattctttccatcccgatgattgtcgcgggtgtgatcatgatggtctgggcatatcgtcgcagcccacagcaacacgtttccttaggaggtggaggtagtggtggaggtggaagtggtggaggtggtagtggtgcagctctggacacgggccagttgctgaagatcgcgaagcggggaggagtcacggcggtcgaggcggtgcacgcgtggcgcaatgcgctcacgggagcacccctcaacctgacagagacgcctgaccccggaacaggtggtggccattgcctccaatattggtggcaagcaagccctggaaaccgttcagcggctgctgccagtcctgtgccaggcacacggcttgacaccagaacaagtggtggcgatcgccagcaataacggcggtaaacaggctctggagactgtgcagcgcttgctcccagtgctgtgtcaggcccacggactccgtctcgactcacgcctgagcaggtagtggctattgcatccaacggagggggcagacccgcactggagtcaatcgtggcccagctttcgaggccggaccccgcgctggcccaccaccaccaccaccactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z