BBa_K1459017 1 BBa_K1459017 pmrC-GFP 2014-10-16T11:00:00Z 2015-05-08T01:10:32Z We obtained pmrC-GFP sequence thanks to kindness of prof. He from the University of Chicago. This is pmrC promoter from Salmonella enterica, with subsequent GFP. This part is one part of PmrA-PmrB detecting system. Upon phosphorylation by PmrB, PmrA binds to pmrC and induces expression of GFP. PmrB gains kinase activities after binding ions by it's extracellular loop (eg. iron or terbium atoms, depending on present binding tag). false false _1837_ 0 20803 9 Not in stock false Apart from amplifying the sequence of interest we had to also perform a directed mutagenesis to eradicate XbaI site from pmrC sequence. false Grzegorz Ścibisz component2424691 1 BBa_E0040 component2424689 1 BBa_K1459006 annotation2424689 1 BBa_K1459006 range2424689 1 1 46 annotation2424691 1 BBa_E0040 range2424691 1 53 772 BBa_K1459006 1 BBa_K1459006 pmrC 2014-10-06T11:00:00Z 2015-05-08T01:10:32Z We obtained the pmrC-GFP sequence thanks to kindness of prof. He from University of Chicago. This is pmrC promoter native to Salmonella enterica. PmrA-PmrB two-component system is native to Salmonella enterica and in it's native state it is responsible for chemotaxis. PmrB is a transmembrane protein with iron binding peptide on it's extracellular loop. When PmrB binds iron (III) iron, it's intracellular domain gains kinase activity and phosphorylates PmrA, which subsequently binds to pmrC promoter and induces expression of chemotaxis CheZ protein. In this part iron binding tag on the extracellular loop was exchanged with a lanthanide binding tag (LBT), to allow PmrA-PmrB two-component system to respond to lanthanide ions. false false _1837_ 0 20803 9 Not in stock false This is pmrC sequence derived from bioinformatic sources, after search for promotor at 5' end of CheZ protein. false Grzegorz Ścibisz BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1459006_sequence 1 tcaccctggtgccttatctttacacctgtgcggcgttactgctgctcggacacggtcactttggtaaagcacgcccggcatatctggcagttactaccattgccttcctctactgcatctgggccgtggtggggtccggagcgaaagaggttatgtggtcatttgtcaccctgatggtcatcaccgccatgtatgcgctgaattacaaccggctacataaaaacccgtatcccttagatgcaccaataagcaaagattaattccccttaatccagcaaacataaaagccaaccttaagaacttaaggttggcttaattttgctttgcgagcatatgcgcactttgttcgatggaaacaccgtggtacccggggatcctctataattaaagaggagaaattaagc BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K1459017_sequence 1 cgatggaaacaccgtggtacccggggatcctctataattaaagaggtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z