LOCUS       BBa_K1461303            1007 bp    DNA     linear       13-Jun-2024
DEFINITION  RBS-GFP-4 miR 142 5p non-binding site-d.term
ACCESSION   BBa_K1461303
VERSION     BBa_K1461303.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     misc_feature    747..769
                     /label=misc
     misc_feature    747..771
                     /label=BBa_K1461103
     misc_feature    780..802
                     /label=misc
     misc_feature    780..804
                     /label=BBa_K1461103
     misc_feature    813..835
                     /label=misc
     misc_feature    813..837
                     /label=BBa_K1461103
     misc_feature    846..868
                     /label=misc
     misc_feature    846..870
                     /label=BBa_K1461103
     stem_loop       890..933
                     /label=stem_loop
     misc_feature    879..958
                     /label=BBa_B0010
     terminator      879..958
                     /label=BBa_B0010
     stem_loop       974..993
                     /label=T7 TE
     misc_feature    1000..1000
                     /label=stop
     polyA_site      994..1007
                     /label=polya
     misc_feature    967..1007
                     /label=BBa_B0012
     terminator      967..1007
                     /label=BBa_B0012
     terminator      879..1007
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagaggtcc ataaagtagg aaacactaca ttactagagg
      781 tccataaagt aggaaacact acattactag aggtccataa agtaggaaac actacattac
      841 tagaggtcca taaagtagga aacactacat tactagagcc aggcatcaaa taaaacgaaa
      901 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      961 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//