LOCUS       BBa_K1462320            2225 bp    DNA     linear       17-May-2024
DEFINITION  pGAL1+PRK+GBD-ligand+tADH1
ACCESSION   BBa_K1462320
VERSION     BBa_K1462320.1  
FEATURES             Location/Qualifiers
     promoter        1..549
                     /label=BBa_J63006
     CDS             556..1764
                     /label=BBa_K1462310
     CDS             1773..1868
                     /label=GBD ligand
     CDS             1773..1868
                     /label=BBa_K1462460
     misc_feature    1877..2225
                     /label=ADH1-Terminator
     terminator      1877..2225
                     /label=BBa_K801012
ORIGIN
        1 ccccattatc ttagcctaaa aaaaccttct ctttggaact ttcagtaata cgcttaactg
       61 ctcattgcta tattgaagta cggattagaa gccgccgagc gggtgacagc cctccgaagg
      121 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
      181 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
      241 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
      301 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
      361 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
      421 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
      481 caaaaaattg ttaatatacc tctatacttt aacgtcaagg aggaaactag acccgccgcc
      541 accatggagt actagatggc tgtttgtact gtctacacaa ttccaactac tactcattta
      601 ggttcttctt tcaatcagaa taataaacaa gtcttcttta attacaaaag atcttcttct
      661 tctaacaata cattgttcac tactagacca tcatatgtca ttacttgttc acaacaacaa
      721 actattgtca ttggtttggc tgctgactct ggttgcggta aatctacttt catgagaaga
      781 ttgacatctg tcttcggtgg tgctgctgaa cctcctaaag gtggtaatcc agattcaaac
      841 acattgattt ctgatacaac tactgttatt tgtttagatg attttcattc tttagataga
      901 aacggtagaa aagtcgaaaa ggttactgct ttggacccaa aagcaaatga cttcgattta
      961 atgtatgaac aagtcaaggc tttgaaggag ggtaaggctg tcgacaagcc aatatacaac
     1021 cacgtctctg gattgttgga cccaccagag ttgattcaac cacctaaaat tttggttatt
     1081 gagggtttgc acccaatgta cgacgcaaga gttagggaat tgttggattt ttcaatatat
     1141 ttggatattt ctaatgaagt taaatttgca tggaaaattc aaagagacat gaaagaaaga
     1201 ggtcactctt tagaatctat taaggcttct attgaaagca ggaaaccaga ttttgatgct
     1261 tacattgacc ctcaaaagca gcacgcagat gtcgtcattg aggtcttgcc aacagaattg
     1321 attcctgatg atgatgaagg taaggttttg agagttagaa tgattcaaaa agaaggtgtt
     1381 aaatttttta acccagttta tttatttgat gaaggttcta ctatttcttg gattccatgt
     1441 ggtagaaaat tgacatgttc atacccaggt attaaattct cttacggtcc agacactttc
     1501 tacggaaacg aggtcacagt cgttgagatg gacggtatgt tcgacaggtt ggacgaatta
     1561 atttacgttg aatctcattt gtctaacttg tctactaaat tttatggtga agtcacacaa
     1621 caaatgttga aacatcaaaa ttttccaggt tcaaataatg gtacaggttt ctttcaaact
     1681 attattggtt tgaaaattag agatttattt gaacaattgg ttgcttctag gtcaacagct
     1741 acagcaacgg cggcgaaagc ttaatactag agttggttgg tgctttgatg cacgtcatgc
     1801 agaagaggtc tagggctatt cactcttctg acgagggtga ggaccaagct ggtgatgaag
     1861 atgaagatta ctagagagct ttggacttct tcgccagagg tttggtcaag tctccaatca
     1921 aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa gatggaaaag ggtcaaatcg
     1981 ttggtagata cgttgttgac acttctaaat aagcgaattt cttatgattt atgattttta
     2041 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2101 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2161 tatagcatga ggtcgctctt attgaccaca cctctaccgg ccggtcgaaa ttcccctacc
     2221 ctatg
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