BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1463041
1
attB
Reversed PhiC31 attB site
2014-08-13T11:00:00Z
2015-05-08T01:10:37Z
Sythesised by IDT Technologies from a given sequence.
PhiC31 attB site
false
false
_1842_
0
23036
9
Not in stock
false
...
false
Beth Greig
BBa_K1463501
1
rev promot
J23100 promoter in reverse
2014-08-11T11:00:00Z
2015-05-08T01:10:37Z
Synthesised by IDT Technologies from a given sequence. Did not come from any genomic sequence.
J23100 Constitutive promoter reversed
false
false
_1842_
0
23036
9
Not in stock
false
No special design considerations other than ensuring the promoter was in reverse.
false
Beth Greig
BBa_K1463002
1
BBa_K1463002
Recombinase Switch With GFP (weaker RBS)
2014-10-14T11:00:00Z
2015-05-08T01:10:36Z
Composite biobrick consisting of attP, reversed BBa_J23100 promoter, forward facing BBa_B0010 terminator, PhiC31 attB site and the BBa_E0040 GFP gene with a medium strength BBa_B0032 ribosome binding site.
PhiC31-based recombinase switch with GFP to the right and a leftward facing promoter so that PhiC31 integrase switches on GFP expression. GFP contains a weaker ribosome binding site than in BBa_K1463001.
false
false
_1842_
0
20827
9
Not in stock
false
The promoter had to face leftwards, the attP and attB sites had to be far enough apart to recombine efficiently and be in head-to-head (inverted repeat) orientation. GFP had to be to the right of the switch.
false
iGEM14_Glasgow
component2420028
1
BBa_B0010
component2420025
1
BBa_K1463040
component2420032
1
BBa_B0032
component2420027
1
BBa_K1463501
component2420035
1
BBa_E0040
component2420030
1
BBa_K1463041
component2420026
1
BBa_K1463030
annotation2420027
1
BBa_K1463501
range2420027
1
82
116
annotation2420032
1
BBa_B0032
range2420032
1
276
288
annotation2420028
1
BBa_B0010
range2420028
1
125
204
annotation2420025
1
BBa_K1463040
range2420025
1
1
54
annotation2420035
1
BBa_E0040
range2420035
1
295
1014
annotation2420026
1
BBa_K1463030
range2420026
1
63
73
annotation2420030
1
BBa_K1463041
range2420030
1
213
267
BBa_K1463030
1
spacer
Switch Nucleotide Spacer
2014-08-13T11:00:00Z
2015-05-08T01:10:36Z
Synthesised by IDT Technologies from a given sequence. Sequence is not from genome.
BamHI and HindIII sites contained when combined with scar sequences in switch, part BBa_K1463000. This spacer is required for the function of this larger part.
false
false
_1842_
0
23036
9
Not in stock
false
Created this part with 2 restriction sites when combined with scar sites in larger composite part.
false
Beth Greig
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_K1463040
1
attP
PhiC31 attP site
2014-08-13T11:00:00Z
2015-05-08T01:10:37Z
Synthesised by IDT technologies from a given sequence.
PhiC31 attP site
false
false
_1842_
0
23036
9
Not in stock
false
...
false
Beth Greig
BBa_K1463040_sequence
1
gagtagtgccccaactggggtaacctttgagttctctcagttgggggcgtaggg
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1463501_sequence
1
gctagcactgtacctaggactgagctagccgtcaa
BBa_K1463030_sequence
1
gatccgaagct
BBa_B0032_sequence
1
tcacacaggaaag
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_K1463041_sequence
1
aggtggagtacgcgcccggggagcccaagggcacgccctggcacccgcaccgcgg
BBa_K1463002_sequence
1
gagtagtgccccaactggggtaacctttgagttctctcagttgggggcgtagggtactagaggatccgaagcttactagaggctagcactgtacctaggactgagctagccgtcaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagaggtggagtacgcgcccggggagcccaagggcacgccctggcacccgcaccgcggtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z