LOCUS       BBa_K1465212            2267 bp    DNA     linear       15-May-2024
DEFINITION  Phosphoribulokinase (prkA) from Synechoccus elongatus under the 
            control of the ptac promoter
ACCESSION   BBa_K1465212
VERSION     BBa_K1465212.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=stop
     CDS             1..1084
                     /label=LacI
     misc_feature    1081..1084
                     /label=start
     RBS             1085..1092
                     /label=rbs
     promoter        1093..1153
                     /label=LacIq promoter
     misc_feature    1..1153
                     /label=BBa_K731300
     misc_feature    1194..1219
                     /label=lacO operator
     promoter        1162..1188
                     /label=Ptac promoter
     promoter        1..1239
                     /label=BBa_K731500
     RBS             1248..1259
                     /label=B0034
     CDS             1266..2267
                     /label=Phosphoribulokinase prkA
     misc_feature    1248..2267
                     /label=BBa_K1465201
ORIGIN
        1 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
       61 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
      121 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
      181 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
      241 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
      301 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
      361 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
      421 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
      481 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
      541 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
      601 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
      661 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
      721 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
      781 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
      841 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
      901 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
      961 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     1021 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     1081 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     1141 tttgcgccat tctactagag gttgacaatt aatcatcggc tcgtataatg tgtgggaatt
     1201 gtgagcggat aacaattccc tcatgaaata attttgtttt actagagaaa gaggagaaat
     1261 actagatgag caagccagat cgtgttgttc tgatcggcgt tgccggtgac tccggttgcg
     1321 gcaagtcaac cttcctaaac cgccttgccg acctgtttgg taccgaactg atgaccgtta
     1381 tctgcctgga tgactatcac tctctggatc gcaagggccg taaggaagca ggcgtaaccg
     1441 ctctggatcc gcgcgccaac aactttgacc tgatgtatga acaggttaag gcgctgaaga
     1501 acggcgaaac catcatgaag ccgatctaca accatgaaac cggcctgatc gatccgccgg
     1561 aaaaaatcga accgaaccgc atcattgtga tcgagggtct gcatccgctt tacgacgagc
     1621 gcgtgcgtga actgctggat ttcagcgttt acctggacat cgatgacgaa gttaaaatcg
     1681 cttggaagat ccaacgcgat atggcagaac gcggccactc ctacgaagat gttctggcct
     1741 ctatcgaagc gcgccgcccg gacttcaagg cctacattga gccgcagcgt ggccatgcgg
     1801 acatcgttat ccgcgttatg ccgacccagc taatcccgaa cgacaccgag cgcaaggtgc
     1861 tgcgtgtgca gctgatccaa cgtgaaggcc gcgatggttt tgagccggct tacctgttcg
     1921 acgaaggttc taccatccag tggaccccgt gcggtcgtaa gctgacatgc tcctatccgg
     1981 gcattcgctt agcctacggc ccggacacct actacggtca cgaagtttca gtgcttgagg
     2041 ttgacggtca gttcgagaac ctggaagaga tgatctacgt tgagggccac ctgagcaaga
     2101 ccgacaccca gtactacggt gagctgaccc acctgctgct gcaacacaaa gattacccgg
     2161 gttctaacaa cggcaccggt ctgttccaag tgctgaccgg cctgaaaatg cgtgcggcct
     2221 atgagcgtct gacctcccaa gcagcaccgg ttgccgctag cgtttaa
//