BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961227
1
start
range1961227
1
173
173
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_K1472610
1
BBa_K1472610
Delta 15 desaturase
2014-09-27T11:00:00Z
2015-05-08T01:10:39Z
The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli.
This sequence encoded for the delta15 desaturase. The gene is from Synechocystis sp. and optimised for the expression in E. coli. During the fatty acid conversion process, delta 15 desaturase catalyses the conversion of linolenic acid (18:2) into alpha-Linolenic acid, ALA (18:3) by adding a cis-doube bond at carbon 15.
false
false
_1851_
0
23007
9
In stock
false
The enzyme cut site must be designed according to iGEM prefix and suffix requirements.
false
Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
annotation2390390
1
Delta 15 desaturase
range2390390
1
1
1104
BBa_K1027001
1
BBa_K1027001
Delta 9 Desaturase, optimised for expression in Escherichia coli
2013-09-18T11:00:00Z
2015-05-08T01:08:46Z
Synthesized gene based off the protein sequence from Synechocystis sp
This enzyme is obtained fro the gene Stearoly Co-A Desaturase (SCD). The gene is obtained from Synechocystis sp., a cyanobacterium.It is involved in the biosynthesize of C18:1 from C18:0. C18:1 corresponds to Oleic acid. The delta 9 desaturase introduces cis carbon double bond on the 9th carbon in C18:0.
false
false
_1334_
0
15689
9
In stock
true
Codon optimized for expression in E. coli
false
Robert Harrison, Lorna Hepworth, Vun Hiang Tan
annotation2357079
1
Stop
range2357079
1
955
960
annotation2357078
1
Start
range2357078
1
1
3
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1027002
1
BBa_K1027002
Delta 12 Desaturase, optimised for expression in Escherichia coli
2013-09-18T11:00:00Z
2015-05-08T01:08:46Z
Produced synthetically based on protein sequence from Synechocystis sp.
This enzyme is obtained from the gene Oleic acid desaturase (ode).The gene is obtained from Synechocystis sp., a cyanobacterium and introduced in the E.coli. It is a trans-membrane enzyme, involved in the biosynthesis of C18:2 from C18:1. C18:2 corresponds to Linolenic acid. The delta 12 desaturase introduces a second cis carbon double bond on the 12th carbon in C18:1.
false
false
_1334_
0
15689
9
In stock
true
Codon optimization for expression in E coli
false
Robert Harrison, Lorna Hepworth, Vun Hiang Tan
annotation2354835
1
Stop
range2354835
1
1057
1062
annotation2354834
1
Start
range2354834
1
1
3
BBa_K1472611
1
BBa_K1472611
IPTG inducible expression of ∆9, ∆12, and ∆15 desaturases
2014-09-27T11:00:00Z
2015-05-08T01:10:39Z
The amino acid sequences of desaturases genes are derived from Synechocystis sp., but codon optimised for expression in E. coli.
It is a device that contained a promoter (BBa_R0010), RBS (BBa_B0034) for each desaturase gene ∆9,12 and ∆15. When free fatty acids, for example, stearic acid (18:0) enters cell, it is converted into oleic acid (18:1∆9) by ∆9 desaturase, then to linolenic acid (18:2∆9,12) by ∆12 desaturase and finally to alpha-Linolenic acid, ALA (18:3∆9,12,15) by ∆15 desaturase. This device is designated to express under the presence of IPTG.
false
false
_1851_
0
23007
9
Not in stock
false
The size of the whole device could be relatively large. The selection of promoter is important as it controls when the machine starts producing desaturases. The investment of producing 3 enzymes in a time could be a energy burden for E. coli.
false
Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu
component2390501
1
BBa_B0034
component2390496
1
BBa_B0034
component2390499
1
BBa_K1027002
component2390491
1
BBa_B0034
component2390494
1
BBa_K1027001
component2390503
1
BBa_K1472610
component2390483
1
BBa_R0010
annotation2390491
1
BBa_B0034
range2390491
1
209
220
annotation2390494
1
BBa_K1027001
range2390494
1
227
1186
annotation2390501
1
BBa_B0034
range2390501
1
2283
2294
annotation2390483
1
BBa_R0010
range2390483
1
1
200
annotation2390499
1
BBa_K1027002
range2390499
1
1213
2274
annotation2390503
1
BBa_K1472610
range2390503
1
2301
3384
annotation2390496
1
BBa_B0034
range2390496
1
1195
1206
BBa_K1472610_sequence
1
atgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat
BBa_K1027001_sequence
1
atgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1027002_sequence
1
atgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatga
BBa_K1472611_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgctgaatccgctgaatattgaatatctgtacctgagcaaactgttcgataatagcctgatcgtgtttaacaaacgtcagctgtttcgtttttttgtgcgcttttttttcatgaccgcagcactgccgaatgatagcaaaccgaaactgacaccggcatggaccgtgatttttttctttaccagcattcatctggttgcactgctggcatttctgccgcagttttttagctggaaagcagttggtatggcctttctgctgtatgttattaccggtggtattggtattaccctgggttttcatcgttgtattagccatcgtagctttaatgttccgaaatggctggaatatatctttgttatttgtggcaccctggcctgtcagggtggtgtttttgaatgggttggtctgcatcgtatgcatcacaaatttagcgataccacaccggatccgcatgatagcaataaaggtttttggtggtcacatattggctggatgatgtttgaaattccggcaaaagcagatattccgcgttataccaaagatatccaggacgataaattctaccagttctgccagaataacctgattctgattcaggttgcactgggtctgattctgtttgccctgggtggttggccgtttgttatttggggtatttttgttcgtctggtgtttgtgtttcactttacctggtttgttaatagcgccacccataaatttggttatgttagccatgagagcaacgattatagccgtaattgttggtgggttgccctgctgacctttggtgaaggttggcataataatcatcacgcctatcagtatagcgcacgtcatggtctgcaatggtgggaagttgatctgacctggatgaccatcaaatttctgagcctgctgggtctggcaaaagatattaaactgcctccggaaaccgcaatggcaaataaagcctaataatactagagaaagaggagaaatactagatgaccgcaaccattccgcctctgaccccgaccgttaccccgagcaatccggatcgtccgattgcagatctgaaactgcaagatattatcaaaaccctgccgaaagaatgcttcgaaaaaaaagcaagcaaagcatgggcaagcgttctgattaccctgggtgcaattgcagttggttatctgggtattatctatctgccgtggtattgtctgccgattacctggatttggaccggcaccgcactgaccggtgcatttgttgttggtcatgattgtggtcatcgtagctttgcaaaaaaacgttgggttaatgatctggtgggtcatattgcatttgcaccgctgatttatccgtttcatagctggcgtctgctgcatgatcatcatcatctgcataccaacaaaatcgaagttgataatgcatgggatccgtggtcagttgaagcatttcaggcaagtccggcaattgttcgtctgttttatcgtgcaattcgtggtccgttttggtggaccggtagcatttttcattggagcctgatgcattttaaactgagcaattttgcacagcgcgatcgcaataaagtgaaactgagcattgcagtggtttttctgtttgcagcaattgcatttccggcactgattattaccaccggtgtttggggttttgtgaaattttggctgatgccgtggctggtttatcatttttggatgagcacctttaccatcgtgcatcataccattccggaaattcgttttcgtccggcagcagattggagcgcagccgaagcacagctgaatggcaccgttcattgtgattatccgcgttgggttgaagttctgtgccatgatattaatgtgcatattccgcatcatctgagcgttgcaattccgagctataatctgcgtctggcacatggtagcctgaaagaaaattggggtccgtttctgtatgaacgcacctttaattggcagctgatgcagcagattagcggtcagtgtcatctgtatgatccggaacatggttatcgtacctttggcagcctgaaaaaagtgtaataatgatactagagaaagaggagaaatactagatgcgtctggaaattagcagtccgcagaccaaactgccgtatccgaaaaccgaagaactgccgtttaccctgcaagaactgcgtaatgcaattccggcagattgttttgaaccgagcgttgttcgtagcctgggttatttctttctggatgttggtctgattgcaggtttttatgcactggcagcatatctggatagctggtttttctatccgattttttggctgattcagggcaccctgttttggagcctgtttgttgttggtcatgattgtggtcatggtagctttagcaaaagcaaaaccctgaataattggattggtcatctgagccataccccgattctggttccgtatcatggttggcgtattagccatcgtacccatcatgcaaataccggtaatattgataccgatgaaagctggtatccggttagcgaacagaaatataaccagatggcatggtatgaaaaactgctgcgtttttatctgccgctgattgcatatccgatttacctgtttcgtcgtagcccgaatcgtcagggtagccattttatgcctggtagtccgctgtttcgtccgggtgaaaaagcagcagttctgaccagcacctttgccctggcagcatttgttggttttctgggctttctgacctggcagtttggttggctgtttctgctgaaattctatgttgcaccgtatctggtttttgttgtttggctggatctggttacctttctgcatcataccgaagataacattccgtggtatcgtggtgatgattggtattttctgaaaggtgcactgagcaccattgatcgtgattatggttttatcaacccgatccatcatgatattggcacccatgttgcacatcatatctttagcaatatgccgcactataaactgcgtcgtgcaaccgaagcaattaaaccgattctgggtgaatattatcgttatagcgacgaaccgatttggcaggcatttttcaaaagctattgggcctgtcattttgttccgaatcagggtagcggtgtttattatcagagcccgagcaatggtggttatcagaaaaaaccgtaataat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z