LOCUS       BBa_K1475005            1603 bp    DNA     linear       10-Jun-2024
DEFINITION  GFP controlled by TetR and pTet.
ACCESSION   BBa_K1475005
VERSION     BBa_K1475005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    51..56
                     /label=BBa_K1088022
     misc_feature    57..59
                     /label=ATG
     misc_feature    678..683
                     /label=TAATAA
     CDS             57..683
                     /label=BBa_K1475003
     misc_feature    684..717
                     /label=B1002
     stem_loop       693..708
                     /label=stem loop
     polyA_site      708..714
                     /label=Poly A tail
     polyA_site      687..692
                     /label=Poly A tail
     terminator      684..717
                     /label=BBa_B1002
     misc_feature    1..717
                     /label=BBa_K1475004
     RBS             726..740
                     /label=RBS-1\Strong
     RBS             733..737
                     /label=RBS
     misc_feature    726..740
                     /label=BBa_B0030
     RBS             726..740
                     /label=BBa_B0030
     CDS             747..1466
                     /label=GFP protein
     CDS             747..1466
                     /label=BBa_E0040
     stem_loop       1486..1529
                     /label=stem_loop
     misc_feature    1475..1554
                     /label=BBa_B0010
     terminator      1475..1554
                     /label=BBa_B0010
     stem_loop       1570..1589
                     /label=T7 TE
     misc_feature    1596..1596
                     /label=stop
     polyA_site      1590..1603
                     /label=polya
     misc_feature    1563..1603
                     /label=BBa_B0012
     terminator      1563..1603
                     /label=BBa_B0012
     misc_feature    726..1603
                     /label=BBa_E0840
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgt
       61 ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa
      121 tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt
      181 attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata
      241 ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata
      301 acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag
      361 gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc
      421 aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt
      481 taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta
      541 ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg
      601 cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta
      661 aatgtgaaag tgggtcctaa taacgcaaaa aaccccgctt cggcggggtt ttttcgctac
      721 tagagattaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
      781 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
      841 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
      901 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
      961 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     1021 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1081 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1141 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     1201 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     1261 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     1321 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     1381 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     1441 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     1501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//