LOCUS       BBa_K1475009            1886 bp    DNA     linear       10-Jun-2024
DEFINITION  Limonene synthase with tet promoter, RBS and terminator
ACCESSION   BBa_K1475009
VERSION     BBa_K1475009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..65
                     /label=SacI
     RBS             66..72
                     /label=rbs
     RBS             63..72
                     /label=BBa_J15001
     CDS             79..1749
                     /label=(+) limonene synthase coding region
     misc_feature    79..81
                     /label=start
     misc_feature    1744..1749
                     /label=stop
     CDS             79..1749
                     /label=BBa_I742110
     misc_feature    63..1749
                     /label=BBa_I742111
     stem_loop       1769..1812
                     /label=stem_loop
     misc_feature    1758..1837
                     /label=BBa_B0010
     terminator      1758..1837
                     /label=BBa_B0010
     stem_loop       1853..1872
                     /label=T7 TE
     misc_feature    1879..1879
                     /label=stop
     polyA_site      1873..1886
                     /label=polya
     misc_feature    1846..1886
                     /label=BBa_B0012
     terminator      1846..1886
                     /label=BBa_B0012
     terminator      1758..1886
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agctcaagga ggtactagat gcgtcgttca gcaaactacc aaccttcaat ttgggaccac
      121 gattttttgc agtcattgaa tagcaactat acggatgaag catacaaaag acgagcagaa
      181 gagctgaggg gaaaagtgaa gatagcgatt aaggatgtaa tcgagcctct ggatcagttg
      241 gagctgattg ataacttgca aagacttgga ttggctcatc gttttgagac tgagattagg
      301 aacatattga ataatatcta caacaataat aaagattata attggagaaa agaaaatctg
      361 tatgcaacct cccttgagtt tagactactt agacaacatg gctatcctgt ttctcaagag
      421 gttttcaatg gttttaaaga cgaccaggga ggcttcattt gtgatgattt caagggaata
      481 ctgagcttgc atgaagcttc gtattacagc ttagaaggag aaagcatcat ggaggaggcc
      541 tggcaattta ccagcaaaca tcttaaagaa gtgatgatca gcaagaacat ggaagaggat
      601 gtatttgtag cagaacaagc gaagcgtgca ctggaactgc ctctgcattg gaaagtgcca
      661 atgttagagg caaggtggtt catacacatt tatgagagaa gagaggacaa gaaccacctt
      721 ttacttgaac tggctaagat ggagtttaac actttgcagg caatttacca ggaagaacta
      781 aaagaaattt cagggtggtg gaaggataca ggtcttggag agaaattgag ctttgcgagg
      841 aacaggttgg tagcgtcctt cttatggagc atggggatcg cgtttgagcc tcaattcgcc
      901 tattgtcgga gagtgctcac aatctcgata gccctaatta cagtgattga tgacatttat
      961 gatgtctatg gaacattgga tgaacttgag atattcactg atgctgttga gaggtgggac
     1021 atcaattatg ctttgaagca ccttccgggc tatatgaaaa tgtgttttct tgcgctttac
     1081 aactttgtta atgaatttgc ttattacgtt ctcaaacaac aggattttga tttgcttctg
     1141 agcataaaaa atgcatggct tggcttaata caagcctact tggtggaggc gaaatggtac
     1201 catagcaagt acacaccgaa actggaagaa tacttggaaa atggattggt atcaataacg
     1261 ggccctttaa ttataacgat ttcatatctt tctggtacaa atccaatcat taagaaggaa
     1321 ctggaatttc tggaaagtaa tccagatata gttcactggt catccaagat tttccgtctg
     1381 caagatgatt tgggaacttc atcggacgag atacagagag gggatgttcc gaaatcaatc
     1441 cagtgttaca tgcatgaaac tggtgcctca gaggaagttg ctcgtcaaca catcaaggat
     1501 atgatgagac agatgtggaa gaaggtgaat gcatacacag ccgataaaga ctctcccttg
     1561 actggaacaa ctactgagtt cctcttgaat cttgtgagaa tgtcccattt tatgtatcta
     1621 catggagatg ggcatggtgt tcaaaaccaa gagactatcg atgtcggttt tacattgctt
     1681 tttcagccca ttcccttgga ggacaaacac atggctttca cagcatctcc tggcaccaaa
     1741 ggctaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1861 ttcgggtggg cctttctgcg tttata
//